SELE Human, HEK

E-Selectin is a heavily glycosylated transmembrane protein. It consists of:

• An N-terminal type 1 lectin domain
• An EGF-like domain
• Six sushi (CCP/SCR) domains
• A transmembrane sequence
• A short cytoplasmic domain

Recombinant human E-selectin is a 58.6kDa protein containing 535 amino acid residues, corresponding to the extracellular portion of the full-length protein. Due to glycosylation, E-selectin migrates at an apparent molecular weight of approximately 65-85kDa by SDS-PAGE analysis under reducing conditions .

E-Selectin plays a crucial role in the recruitment of circulating leukocytes from blood to sites of inflammation in the vascular lining through interaction with specific cell surface-associated carbohydrate determinants . It is a pro-angiogenic factor and has been implicated in the pathogenesis of stroke and atherosclerosis .

The recombinant form of E-Selectin is expressed in HEK 293 cells. HEK (Human Embryonic Kidney) cells are commonly used for the production of recombinant proteins due to their high transfection efficiency and ability to perform post-translational modifications similar to those in human cells .

E-Selectin is used in various research applications, including:

• Studying the mechanisms of leukocyte recruitment and inflammation
• Investigating the role of E-Selectin in angiogenesis and vascular diseases
• Developing therapeutic strategies targeting E-Selectin for inflammatory and cardiovascular diseases

Recombinant E-Selectin is typically lyophilized from a 0.22 μm filtered solution in PBS, pH 7.4, with mannitol or trehalose added as protectants before lyophilization. It should be reconstituted in sterile PBS, pH 7.4, to a concentration of 50 μg/mL and stored at 2-8°C for up to one month or at -20°C for extended storage .

E-Selectin is a vital molecule in the study of inflammation and vascular biology, providing insights into the mechanisms of leukocyte recruitment and the development of therapeutic interventions for related diseases.