Dengue NS1, ST3
Description
Product Specs
Purified by proprietary chromatographic technique
Product Science Overview
Dengue virus (DENV) is a mosquito-borne virus that belongs to the Flaviviridae family. It is responsible for causing dengue fever, a severe flu-like illness that affects millions of people worldwide. The virus has four distinct serotypes: DENV1, DENV2, DENV3, and DENV4. Each serotype can cause dengue fever, and infection with one serotype does not provide immunity against the others .
The dengue virus genome encodes ten proteins, including three structural proteins (capsid, pre-membrane, and envelope) and seven non-structural proteins (NS1, NS2A, NS2B, NS3, NS4A, NS4B, and NS5) . Among these, the non-structural protein 1 (NS1) plays a crucial role in the virus’s life cycle and pathogenesis. NS1 is a glycoprotein that is involved in viral replication and immune evasion. It is secreted from infected cells and can be detected in the blood of infected individuals .
Recombinant NS1 proteins are produced using genetic engineering techniques to express the NS1 protein in various host systems, such as bacteria, yeast, or mammalian cells. These recombinant proteins are used for diagnostic purposes and vaccine development. The expression of recombinant NS1 protein in bacterial systems, such as E. coli, is particularly advantageous due to its simplicity and cost-effectiveness .
The NS1 protein of dengue virus subtype 3 (DENV3) has been extensively studied for its diagnostic and therapeutic potential. Researchers have successfully developed methods to express and purify recombinant DENV3 NS1 protein in E. coli. The gene encoding NS1 is cloned into an expression vector, and the recombinant protein is produced in bacterial cells. The protein is then purified and refolded to attain its native conformation .
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Diagnostic Applications: Recombinant NS1 protein is used in serodiagnostic assays to detect dengue virus infection. These assays are based on the detection of NS1 antigen in the blood of infected individuals. The use of recombinant NS1 protein in diagnostic tests offers a safer and more cost-effective alternative to traditional methods that rely on tissue culture-grown viral proteins .
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Vaccine Development: Recombinant NS1 protein is also being explored as a potential vaccine candidate. The protein can be formulated with adjuvants to enhance its immunogenicity and provide better protection against dengue virus infection. Various strategies, such as protein engineering and fusion with immunostimulatory motifs, are being employed to improve the efficacy of NS1-based vaccines .
