Dengue NS1 Paired Antibody

Mouse Anti Dengue NS1 Paired
Cat. No.
BT6120
Source
Synonyms
Appearance
2 vials of sterile Filtered clear colorless solution.
Purity
Greater than 95%.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

A paired monoclonal antibody has been developed for dengue NS1 antigen lateral flow immunoassay product. It is suggested to use 10µg/ml gold concentration (OD 1.2-1.5) to conjugate with clone 24/c, and 1.2µg/cm coating concentration for clone 2/b. The sensitivity is directly proportional to conjugated gold OD. The specimen used for the test is 80-90 µl. The rapid test developed by these 2 paired antibodies has 90% sensitivity for the samples with OD over 5 and 60% sensitivity for the sample with OD below 5 tested by ELISA.
Please note that when ordering for example: 100µg antibody we ship 50µg from each of the antibodies (100µg in total).

Product Specs

Introduction
Dengue NS1 is an early marker for dengue infection detectable from the first day of fever and lasting for about a week. It can be detected in both primary and secondary infections for all four dengue subtypes. Dengue IgM, another marker, is usually detectable between the first and fifth day of infection.
Description
This product contains a pair of monoclonal antibodies specifically designed for use in a dengue NS1 antigen lateral flow immunoassay. For optimal results, it is recommended to use a gold concentration of 10 µg/ml (with an OD of 1.2-1.5) for conjugation with the clone 24/c antibody. The suggested coating concentration for the clone 2/b antibody is 1.2 µg/cm. It's important to note that the sensitivity of the assay is directly proportional to the optical density of the conjugated gold. The test typically requires a sample volume of 80-90 µl. When tested using samples with an OD over 5, the rapid test developed with these paired antibodies demonstrated a sensitivity of 90%. The sensitivity decreased to 60% for samples with an OD below 5 when analyzed by ELISA. It is important to note that when ordering a specific quantity, for example, 100 µg of antibody, the shipment will contain 50 µg of each antibody, totaling 100 µg.
Purity
The purity of this product is greater than 95%.
Physical Appearance
This product consists of two vials containing a sterile, filtered, clear, and colorless solution.
Formulation
This product includes two antibodies: - Dengue NS1 Conjugation antibody (clone 24/c) supplied at a concentration of 1.2 mg/ml in a solution of PBS and Sodium nitrate. - Dengue NS1 Coating antibody (clone 2/b) supplied at a concentration of 3.4 mg/ml in a solution of PBS and Sodium nitrate.
Stability
To ensure optimal product stability, the Dengue NS1 Paired Antibody should be stored at a constant temperature of 4°C.
Applications
This product is specifically designed for use in lateral flow immunoassays.
Assay Conditions
The following assay conditions are recommended for optimal product performance: 1. Use freshly prepared gold particles (within 1 to 2 days) with maximum homogeneity in particle size. The wavelength should be between 525 and 5529 nm, and the optical density should be between 1.2 and 1.4. 2. Maintain a pH of 8.5 during the conjugation process, which should be carried out for 6-8 hours. 3. For blocking, adjust the optical density of the re-suspended anti-dengue NS1 conjugated colloidal gold to between 8.0 and 8.2. Add casein to achieve a final concentration of 0.3-0.4%.
Purification Method

Purified monoclonal IgG by protein A chromatography.

Type
Mouse antibody Monoclonal.

Product Science Overview

Introduction

Dengue fever, caused by the dengue virus, is a significant global health concern, affecting millions of people annually. The virus is transmitted by Aedes mosquitoes and can lead to severe symptoms, including high fever, severe headache, pain behind the eyes, joint and muscle pain, rash, and mild bleeding. One of the critical components in the diagnosis and study of dengue virus is the non-structural protein 1 (NS1). Mouse anti-Dengue NS1 paired antibodies are essential tools in the detection and research of this protein.

Dengue Virus and NS1 Protein

The dengue virus belongs to the Flaviviridae family and has four distinct serotypes (DENV-1, DENV-2, DENV-3, and DENV-4). The NS1 protein is a non-structural glycoprotein produced by all flaviviruses, including dengue. It plays a crucial role in viral replication and immune evasion. NS1 exists in multiple forms: as a monomer, dimer, and hexamer, each with different functions and locations within the host cell and extracellular environment .

Importance of NS1 in Dengue Diagnosis

NS1 is a significant marker for early dengue infection. It is secreted into the bloodstream during the acute phase of the disease, making it a valuable target for diagnostic assays. Detection of NS1 can be achieved through various methods, including enzyme-linked immunosorbent assays (ELISA) and lateral flow immunoassays (LFIA). These assays rely on the use of specific antibodies that can recognize and bind to the NS1 protein .

Mouse Anti-Dengue NS1 Paired Antibodies

Mouse anti-Dengue NS1 paired antibodies are monoclonal antibodies developed to target the NS1 protein of the dengue virus. These antibodies are typically used in pairs for sandwich ELISA and other immunoassays. One antibody (capture antibody) binds to the NS1 protein, and the other (detection antibody) binds to a different epitope on the same protein, allowing for highly specific and sensitive detection .

Development and Applications

The development of mouse anti-Dengue NS1 paired antibodies involves immunizing mice with recombinant NS1 protein, followed by hybridoma technology to produce monoclonal antibodies. These antibodies are then screened for their ability to bind to NS1 and paired based on their performance in immunoassays .

Applications of these paired antibodies include:

  • Diagnostic Assays: Used in ELISA and LFIA for the early detection of dengue infection.
  • Research: Studying the role of NS1 in dengue pathogenesis and immune response.
  • Vaccine Development: Evaluating the efficacy of dengue vaccines by measuring NS1 levels.

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