Dengue NS1 ST4, Insect
Description
Dengue NS1 ST4 is purified by proprietary chromatographic technique.
Product Specs
Product Science Overview
Dengue virus (DENV) is a mosquito-borne virus that causes dengue fever, a significant public health concern in tropical and subtropical regions. The virus has four distinct serotypes (DENV-1 to DENV-4), each capable of causing disease. One of the critical components of the dengue virus is the nonstructural protein 1 (NS1), which plays multiple roles in the viral life cycle and pathogenesis.
NS1 is a multifunctional protein that is actively secreted by infected cells, including both vertebrate and mosquito cells . It exists in various forms: intracellular, membrane-associated, and secreted. The secreted form of NS1 is particularly significant as it can interact with the host immune system and contribute to the pathogenesis of dengue virus infection .
NS1 is known to activate immune cells via Toll-like receptor 4 (TLR4), leading to the release of proinflammatory cytokines and chemokines . This activation can disrupt endothelial cell monolayer integrity, contributing to the vascular leak observed in severe dengue cases . Additionally, NS1-specific antibodies can mediate antibody-dependent cellular cytotoxicity (ADCC), providing a protective effect in secondary DENV infections .
Recombinant NS1 protein is produced using various expression systems, including insect cells. The use of insect cells, such as Drosophila S2 cells, allows for the production of highly purified NS1 protein devoid of bacterial endotoxin activity . This recombinant protein is valuable for research and vaccine development, as it can be used to study the immune response to NS1 and its role in dengue pathogenesis.
Recombinant NS1 protein has several applications in dengue research. It is used to investigate the mechanisms of NS1-mediated immune activation and vascular leak. Studies have shown that NS1 can act as a viral toxin, similar to bacterial endotoxins, and contribute to the severity of dengue infection . Understanding these mechanisms is crucial for developing targeted therapies and vaccines to mitigate the impact of dengue virus.
Furthermore, recombinant NS1 protein is used in diagnostic assays to detect NS1-specific antibodies in patient samples. These assays can help identify individuals with previous dengue infections and assess their risk of severe disease upon subsequent infections .
