Dengue NS1 n
Description
Product Specs
Dengue protein is purified by proprietary chromatographic technique.
Product Science Overview
Dengue virus (DENV) is a mosquito-borne flavivirus that causes dengue fever, a significant public health concern in tropical and subtropical regions. The virus has four distinct serotypes (DENV-1 to DENV-4), and infection with one serotype provides lifelong immunity to that serotype but not to the others. Dengue virus nonstructural protein 1 (NS1) is a multifunctional glycoprotein that plays a crucial role in the viral life cycle and pathogenesis.
NS1 is a 46-55 kDa glycoprotein that exists in multiple forms: intracellular, membrane-associated, and secreted. It is highly conserved among flaviviruses and is essential for viral replication. NS1 is involved in various functions, including:
- Viral replication: NS1 interacts with other viral nonstructural proteins to form the replication complex.
- Immune evasion: NS1 helps the virus evade the host immune response by interfering with complement activation and antibody-dependent cellular cytotoxicity (ADCC).
- Pathogenesis: Secreted NS1 can induce endothelial cell dysfunction, leading to increased vascular permeability and contributing to the severe manifestations of dengue, such as dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS).
Recombinant NS1 (rNS1) is produced using various expression systems, such as bacterial, yeast, insect, and mammalian cells. The recombinant protein retains the immunogenic properties of the native NS1 and is used in research and diagnostic applications. rNS1 is also being explored as a potential vaccine candidate and therapeutic target.
NS1 is highly immunogenic, and antibodies against NS1 are generated during natural infection. These antibodies can be both protective and pathogenic. Protective antibodies can neutralize the virus and mediate ADCC, while pathogenic antibodies can enhance viral infection through antibody-dependent enhancement (ADE). Studies have shown that NS1-specific antibodies can provide cross-protection against different DENV serotypes .
Recombinant NS1 is widely used in diagnostic assays for dengue infection. NS1 antigen detection tests are valuable for early diagnosis, as NS1 is detectable in the blood during the acute phase of infection. Additionally, rNS1 is being investigated as a vaccine candidate. Preclinical studies have demonstrated that immunization with rNS1 can induce protective immunity and reduce viral load in animal models .
