CST3 Mouse

Cystatin-C Mouse Recombinant
Cat. No.
BT23715
Source
Escherichia Coli.
Synonyms
Post G-globulin, CST 3, CST3, Gamma-Trace, Cystatin 3, Amyloid Angiopathy and Cerebral Hemorrhage, Cystatin-C precursor, neuroendocrine basic polypeptide.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
Quick Inquiry

Description

Cystatin-C Murine Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 134 amino acids and having a molecular mass of 15kDa.
The Mouse Cystatin-C is fused to His tag at N-Terminus.
The Mouse Cystatin-C is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Cystatins are a large family of cysteine protease inhibitors present in various organisms. They are known for their ability to inhibit cysteine proteases, such as cathepsin B, H, L, and S, by forming tight complexes. Cystatin C, a secreted member of this family, holds significance in biochemistry, medicine, and evolutionary studies. This 13260 Da protein, consisting of 120 amino acids, lacks carbohydrate modifications and possesses two disulfide bridges near its carboxyl terminus. Elevated levels of Cystatin C are observed in individuals with malignancies and are associated with impaired kidney function, making it a potentially more reliable marker than creatinine. Conversely, reduced Cystatin C levels can lead to the degradation of elastic laminae, increasing the risk of atherosclerosis and abdominal aortic aneurysms.
Description
Recombinant Murine Cystatin-C, expressed in E. coli, is a single, non-glycosylated polypeptide chain with a molecular weight of 15 kDa, comprising 134 amino acids. This protein is engineered with an N-terminal His tag to facilitate purification. The purification process involves proprietary chromatographic techniques to obtain highly pure Mouse Cystatin-C.
Physical Appearance
White, lyophilized (freeze-dried) powder, sterile-filtered for purity.
Formulation
The protein was concentrated to 0.5mg/ml, sterile filtered, and then lyophilized in a buffer consisting of 20mM Tris and 50mM NaCl at pH 7.5.
Solubility
To prepare a working solution, add deionized water to the lyophilized pellet to achieve a final concentration of 0.5mg/ml. Ensure complete dissolution of the pellet.
Stability
For long-term storage, keep the lyophilized protein at -20°C. After reconstitution, aliquot the protein solution to minimize freeze-thaw cycles. Reconstituted protein can be stored at 4°C for a limited period.
Purity
The purity of the Mouse Cystatin-C is greater than 95% as assessed by SDS-PAGE analysis.
Synonyms
Post G-globulin, CST 3, CST3, Gamma-Trace, Cystatin 3, Amyloid Angiopathy and Cerebral Hemorrhage, Cystatin-C precursor, neuroendocrine basic polypeptide.
Source
Escherichia Coli.
Amino Acid Sequence
MRGSHHHHHH GMASATPKQG PRMLGAPEEA DANEEGVRRA LDFAVSEYNK GSNDAYHSRA IQVVRARKQL VAGVNYFLDV EMGRTTCTKS QTNLTDCPFH DQPHLMRKAL CSFQIYSVPW KGTHSLTKFSCKNA.

Product Science Overview

Introduction

Cystatin-C is a member of the cystatin superfamily, specifically belonging to family 2 of this group. It is a potent inhibitor of cysteine proteases, which are enzymes that degrade proteins by cleaving peptide bonds. Cystatin-C is involved in various physiological and pathological processes, including tumor invasion, metastasis, inflammation, and certain neurological diseases .

Structure and Properties

Recombinant Mouse Cystatin-C is typically produced in a mouse myeloma cell line (NS0) and has a molecular weight of approximately 15 kDa. The protein is often tagged with a C-terminal 10-His tag to facilitate purification and detection . The recombinant form is highly pure, with a purity greater than 95% as determined by SDS-PAGE and visualized with silver staining .

Biological Functions

Cystatin-C inhibits a wide range of cysteine proteases, including papain and cathepsins B, H, K, L, and S . By inhibiting these proteases, Cystatin-C plays a crucial role in regulating proteolytic activity in various tissues and biological fluids. This regulation is essential for maintaining tissue homeostasis and preventing excessive protease activity that can lead to tissue damage.

Expression and Tissue Distribution

Cystatin-C is expressed in all human nucleated cells and is found in high concentrations in biological fluids such as blood, cerebrospinal fluid, and urine . Its expression is regulated by various factors, including inflammatory cytokines and growth factors. Changes in Cystatin-C levels have been documented in several diseases, including cardiovascular diseases, renal failure, and neurodegenerative disorders .

Clinical Significance

Due to its low molecular weight and stable expression, Cystatin-C is considered a reliable biomarker for renal function. It is used as an alternative to creatinine for estimating glomerular filtration rate (GFR) in patients with chronic kidney disease. Elevated levels of Cystatin-C have also been associated with an increased risk of cardiovascular events and mortality .

Recombinant Production and Applications

Recombinant Mouse Cystatin-C is produced using advanced biotechnological methods to ensure high purity and activity. It is used in various research applications, including enzyme inhibition studies, protein-protein interaction assays, and as a standard in ELISA and other immunoassays . The recombinant form is available in both carrier-free and BSA-containing formulations, depending on the specific application requirements .

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

Related Products

CST3 Human
Cystatin-C Human
Cat. No.
BT23409
Source
Human urine.
CST3 k9
Cystatin-C Canine Recombinant
Cat. No.
BT23641
Source
E. coli
CST3 Protein, His
Cystatin-C Human Recombinant, His Tag
Cat. No.
BT24012
Source
Escherichia Coli.
CST3 Rat, sf9
Cystatin C Rat Recombinant, sf9
Cat. No.
BT24318
Source
Sf9, Baculovirus cells.
THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.