CST3 Protein, His

Cystatin-C Human Recombinant, His Tag
Cat. No.
BT24012
Source
Escherichia Coli.
Synonyms
Cystatin-C, Cystatin-3, Neuroendocrine basic polypeptide, Gamma-trace, Post-gamma-globulin, CST3, MGC117328.
Appearance
Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95% as determined by SDS PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Cystatin-C Human Recombinant produced in E.Coli is a single, non-glycosylated, Polypeptide chain containing 129 amino acids and having a molecular mass of 14.5 kDa. The protein contains an extra His tag at N-terminus. The Cystatin-C amino acid sequence is identical to UniProtKB/Swiss-Prot entry Q6FGW9 amino acids 28–146.
The Cystatin-C is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Cystatins are a large family of cysteine protease inhibitors found in various organisms, including plants and animals. These inhibitors play a crucial role in regulating the activity of cysteine proteases, such as cathepsin B, H, L, and S, by forming strong complexes with them. Cystatin C, a secreted protein belonging to this family, has garnered significant interest in the fields of biochemistry, medicine, and evolutionary biology. With a molecular weight of 13260 Da, Cystatin C consists of 120 amino acids and lacks carbohydrate moieties. It features two disulfide bridges located near its carboxyl terminus. Elevated levels of Cystatin C are observed in patients with malignant diseases and are associated with impaired renal function. In fact, Cystatin C is considered a more reliable marker of kidney function than creatinine. Conversely, low levels of Cystatin C can lead to the degradation of elastic laminae, potentially resulting in conditions such as atherosclerosis and abdominal aortic aneurysm.
Description
Recombinant Human Cystatin-C, expressed in E. coli, is a single, non-glycosylated polypeptide chain comprising 129 amino acids, with a molecular weight of 14.5 kDa. This protein variant includes an additional His tag at its N-terminus. The amino acid sequence of this Cystatin-C is identical to amino acids 28-146 of the sequence corresponding to UniProtKB/Swiss-Prot entry Q6FGW9. The purification process of Cystatin-C involves proprietary chromatographic methods.
Physical Appearance
White lyophilized (freeze-dried) powder, filtered for purity.
Formulation
The protein is filtered through a 0.4 μm filter and lyophilized from a solution containing 0.5 mg/ml Cystatin-C in 20 mM Tris buffer (pH 7.5) and 50 mM NaCl.
Solubility
To prepare a working stock solution of approximately 0.5 mg/ml, add deionized water to the lyophilized pellet and allow it to dissolve completely. Note: This product is not sterile. Before using in cell culture, filter the solution through an appropriate sterile filter.
Stability
Store the lyophilized protein at -20°C. After reconstitution, aliquot the protein to minimize repeated freeze-thaw cycles and store at -80°C for long-term storage. The reconstituted protein can be stored at 4°C for a limited period; no significant changes are observed after two weeks at 4°C.
Purity
The purity is determined to be greater than 95% by SDS-PAGE analysis.
Synonyms
Cystatin-C, Cystatin-3, Neuroendocrine basic polypeptide, Gamma-trace, Post-gamma-globulin, CST3, MGC117328.
Source
Escherichia Coli.

Product Science Overview

Introduction

Cystatin-C is a member of the cystatin superfamily, specifically family 2, which consists of cysteine protease inhibitors. This protein is ubiquitous in human tissues and body fluids and is primarily used as a biomarker for kidney function. The recombinant form of Cystatin-C, tagged with a histidine (His) tag, is commonly used in research to study its properties and interactions.

Structure and Properties

Cystatin-C is a small protein with a molecular weight of approximately 14.4 kDa. The recombinant form, expressed in human cells and tagged with a His tag, typically has a slightly higher observed molecular weight due to the tag and other post-translational modifications, ranging from 15 to 18 kDa . The His tag facilitates purification and detection of the protein in various experimental setups.

Function

Cystatin-C functions as an inhibitor of several cysteine proteases, including papain and cathepsins B, H, K, L, and S . By inhibiting these proteases, Cystatin-C plays a crucial role in regulating proteolytic activity within tissues. This regulation is essential for maintaining tissue homeostasis and preventing excessive protease activity that could lead to tissue damage.

Clinical Significance

Cystatin-C is widely recognized as a reliable biomarker for assessing kidney function. Unlike creatinine, another common biomarker, Cystatin-C levels are less influenced by factors such as muscle mass, age, and sex. This makes it a more accurate indicator of glomerular filtration rate (GFR), particularly in individuals with varying muscle mass .

In addition to its role in kidney function, Cystatin-C has been studied for its potential involvement in cardiovascular diseases and neurodegenerative disorders. Elevated levels of Cystatin-C have been associated with an increased risk of cardiovascular events and may serve as a predictor for the onset or progression of conditions such as Alzheimer’s disease .

Applications in Research

The recombinant form of Cystatin-C, particularly the His-tagged version, is extensively used in research to study its biochemical properties and interactions with other proteins. The His tag allows for easy purification using nickel affinity chromatography, and the recombinant protein can be used in various assays to investigate its inhibitory activity against cysteine proteases .

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