CST3 k9

Cystatin-C Canine Recombinant
Cat. No.
BT23641
Source
E. coli
Synonyms
Cystatin-C, Cystatin-3, Neuroendocrine basic polypeptide, Gamma-trace, Post-gamma-globulin, CST3, MGC117328.
Appearance
Purity
Purity is greater than 95% as visualized by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Cystatin-C Protein is a 14.85 kDa protein containing 133 amino acids fused to a 8 aa N-Terminal His-tag.

Product Specs

Introduction
Cystatins belong to a superfamily of cysteine proteinase inhibitors present in various organisms, including plants and animals. This group of inhibitors is widely distributed throughout body fluids and tissues. Cystatins exhibit the ability to form stable complexes with cysteine proteases such as cathepsin B, H, L, and S. Among them, Cystatin C, a secreted protein, holds particular interest in biochemistry, medicine, and evolutionary studies. With a molecular weight of 13,260 Da, Cystatin C consists of 120 amino acids, lacks carbohydrate moieties, and possesses two disulfide bridges near its carboxyl terminus. Elevated levels of Cystatin C are observed in patients with malignancies and are associated with impaired renal function, suggesting its potential as a more sensitive marker than creatinine. Conversely, low Cystatin C levels can lead to the degradation of elastic laminae, potentially contributing to atherosclerosis and abdominal aortic aneurysms.
Description
Cystatin-C Protein from Canis lupus familiaris (dog) is a 14.85 kDa protein comprising 133 amino acids, including an 8 amino acid N-terminal His-tag.
Formulation
Canine Cystatin-C was sterile filtered at 0.4 µm and subsequently lyophilized from a 0.5 mg/ml solution. The lyophilized protein is supplied in a buffer consisting of 20mM TRIS, 50mM NaCl, with a pH of 7.5.
Solubility
To create a working stock solution with a concentration of 0.5 mg/ml, add deionized water to the lyophilized pellet and ensure complete dissolution. It is important to note that the product is not sterile. Prior to using in cell culture, it is essential to sterilize the product by filtration using an appropriate sterile filter.
Stability
Lyophilized K9 Cystatin-C should be stored at -20°C. After reconstituting the product, it is recommended to aliquot and store it at 4°C to minimize repeated freeze-thaw cycles. Reconstituted Cystatin-C remains stable at 4°C for a limited period, showing no detectable changes for up to two weeks.
Purity
The purity of this protein is confirmed to be greater than 95% as determined by SDS-PAGE analysis.
Synonyms
Cystatin-C, Cystatin-3, Neuroendocrine basic polypeptide, Gamma-trace, Post-gamma-globulin, CST3, MGC117328.
Source
E. coli
Amino Acid Sequence
MKHHHHHHAS PGAGRRGSRP GAVGGAVDAD VGEEGVQQAL DFAVREYNRA SNDAYHSRAL RVLRARKQVV SGMNYFLEVE IGRTRCTKSQ PNLDNCPFHD QPHLMRKTLC SFQIYTVPWL GKTSLVKSSC QDV.

Product Science Overview

Introduction

Cystatin-C is a cysteine protease inhibitor that plays a crucial role in regulating proteolytic activity in various biological processes. It is a member of the cystatin superfamily and is known for its ability to inhibit cysteine proteases such as cathepsins. In canines, Cystatin-C is an important biomarker for kidney function and other physiological conditions.

Structure and Properties

Cystatin-C is a small protein with a molecular weight of approximately 14.85 kDa, consisting of 133 amino acids. The recombinant form of Cystatin-C for canines is often produced using E. coli expression systems . This recombinant protein is typically fused with an 8 amino acid N-terminal His-tag to facilitate purification and detection .

The protein is highly purified, with a purity greater than 95% as visualized by SDS-PAGE . It is lyophilized from a solution containing 20mM TRIS and 50mM NaCl at pH 7.5 . The lyophilized form is stable at -20°C and can be reconstituted for use in various assays and experiments .

Biological Function

Cystatin-C inhibits cysteine proteases, which are enzymes that break down proteins by cleaving peptide bonds. By inhibiting these proteases, Cystatin-C helps regulate protein turnover and prevents excessive proteolysis. This regulation is essential for maintaining cellular homeostasis and preventing tissue damage.

In canines, Cystatin-C is a valuable biomarker for assessing kidney function. Elevated levels of Cystatin-C in the blood can indicate impaired kidney function, making it a useful diagnostic tool for veterinarians.

Applications

Recombinant Cystatin-C is widely used in research and diagnostic applications. Some of the key applications include:

  1. Kidney Function Assessment: Measuring Cystatin-C levels in blood samples to evaluate kidney function in canines.
  2. Protease Inhibition Studies: Investigating the role of cysteine proteases in various biological processes and diseases.
  3. Biomarker Research: Exploring the potential of Cystatin-C as a biomarker for other physiological and pathological conditions.

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