CST3 Rat, sf9
Cystatin C Rat Recombinant, sf9
Cat. No.
BT24318
Source
Sf9,
Baculovirus cells.
Synonyms
Cystatin-C, Cystatin-3.
Appearance
Sterile
filtered colorless solution.
Purity
Greater
than 95.0% as determined by SDS-PAGE.
Usage
THE BioTeks products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
CST3 Rat Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 128 amino acids (21-140a.a.) and having a molecular mass of 14.3kDa (Molecular size on SDS-PAGE will appear at approximately 13.5-18kDa).
CST3 is expressed with an 8 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques
Product Specs
Introduction
Cystatins are a family of proteins that inhibit cysteine proteinases. These inhibitors are found in various tissues and fluids throughout the body and play a crucial role in regulating the activity of cysteine proteases like cathepsin B, H, L, and S. Among them, Cystatin C stands out due to its significance in biochemistry, medicine, and evolutionary studies. This secreted molecule, with a molecular weight of 13260 Da and consisting of 120 amino acids, is devoid of carbohydrates and possesses two disulfide bridges near its carboxyl terminus. Elevated levels of Cystatin C are observed in patients with malignant diseases and are associated with impaired kidney function, suggesting its potential as a more reliable marker than creatinine for renal function assessment. Conversely, low levels of Cystatin C can lead to the degradation of elastic laminae, potentially contributing to atherosclerosis and abdominal aortic aneurysm.
Description
Recombinant Rat CST3, produced in Sf9 insect cells using a baculovirus expression system, is a single, glycosylated polypeptide chain. This protein consists of 128 amino acids (spanning from amino acid positions 21 to 140a.a.) and has a molecular mass of 14.3 kDa. On SDS-PAGE, the apparent molecular size will appear between 13.5 kDa and 18 kDa. The CST3 protein is engineered with an 8 amino acid His tag at the C-terminus to facilitate purification, which is carried out using proprietary chromatographic techniques.
Physical Appearance
The product is a clear, colorless solution that has been sterilized by filtration.
Formulation
The CST3 protein is supplied as a solution with a concentration of 0.5 mg/ml. The solution is buffered with Phosphate Buffered Saline (pH 7.4) and contains 10% glycerol to enhance stability.
Stability
For short-term storage (up to 4 weeks), the CST3 protein solution can be stored at 4°C. For extended storage, it is recommended to store the protein in frozen aliquots at -20°C. To ensure long-term stability during frozen storage, it is advisable to add a carrier protein such as HSA or BSA (0.1% final concentration). Repeated freezing and thawing of the protein should be avoided to maintain protein integrity and activity.
Purity
The purity of CST3 Rat Recombinant is determined to be greater than 95% using SDS-PAGE analysis.
Synonyms
Cystatin-C, Cystatin-3.
Source
Sf9,
Baculovirus cells.
Amino Acid Sequence
GTSRPPPRLL
GAPQEADASE EGVQRALDFA VSEYNKGSND AYHSRAIQVV RARKQLVAGI NYYLDVEMGR TTCTKSQTNL
TNCPFHDQPH LMRKALCSFQ IYSVPWKGTH TLTKSSCKNA LEHHHHHH.
Product Science Overview
Introduction
Cystatin C is a cysteine protease inhibitor that plays a crucial role in regulating proteolytic activity in various physiological processes. It is a member of the cystatin superfamily and is known for its ability to inhibit cysteine proteases such as cathepsins. Cystatin C is widely used as a biomarker for kidney function and has been studied extensively in the context of chronic kidney disease (CKD).
Structure and Production
The rat recombinant Cystatin C (sf9) is produced in Sf9 Baculovirus cells. This recombinant protein is a single, glycosylated polypeptide chain containing 128 amino acids (21-140a.a.) and has a molecular mass of approximately 14.3 kDa . The molecular size on SDS-PAGE appears at approximately 13.5-18 kDa .
Function and Significance
Cystatin C is an important biomarker for renal function due to its consistent production rate and low molecular weight, which allows it to be freely filtered by the glomerulus. Unlike creatinine, Cystatin C levels are not significantly influenced by muscle mass, making it a more reliable indicator of glomerular filtration rate (GFR) and kidney function .
Applications in Research and Medicine
- Kidney Function Assessment: Cystatin C is used to estimate GFR and diagnose CKD. It provides a more accurate assessment of renal function compared to creatinine-based methods, especially in individuals with varying muscle mass .
- Cardiovascular Risk Prediction: Elevated levels of Cystatin C have been associated with an increased risk of cardiovascular events and mortality. It is used as a predictive marker for cardiovascular diseases in patients with and without CKD .
- Neurological Disorders: Cystatin C has been implicated in neurodegenerative diseases such as Alzheimer’s disease. It is believed to play a protective role by inhibiting cysteine proteases that contribute to neuronal damage.
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