CST3 Mouse, sf9

Cystatin-C Mouse Recombinant, sf9
Cat. No.
BT23917
Source
Sf9, Insect cells.
Synonyms
Cystatin-C, Cystatin-3, Cst3.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage

THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Description

CST3 produced in Sf9 Insect cells is a single, glycosylated polypeptide chain containing 126 amino acids (21-140a.a.) and having a molecular mass of 14.2kDa. (Molecular size on SDS-PAGE will appear at approximately 13.5-18kDa).
CST3 is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
Cystatins are a family of proteins that inhibit cysteine proteinases. These inhibitors are found in various tissues and fluids throughout the body and play a crucial role in regulating the activity of proteases like cathepsin B, H, L, and S. Among them, Cystatin C stands out due to its significance in biochemistry, medicine, and evolutionary studies. This secreted molecule, with a molecular weight of 13260 Da, consists of 120 amino acids, lacks carbohydrate modifications, and possesses two disulfide bridges near its carboxyl terminus. Elevated levels of Cystatin C are observed in patients with malignant diseases and are linked to impaired kidney function, making it a potentially more reliable marker than creatinine for assessing renal function. Conversely, decreased Cystatin C levels are implicated in the degradation of elastic laminae, which can contribute to conditions like atherosclerosis and abdominal aortic aneurysm.
Description
CST3, produced in Sf9 insect cells, is a single, glycosylated polypeptide chain with a molecular weight of 14.2 kDa, comprising 126 amino acids (21-140a.a.). Its apparent molecular size on SDS-PAGE is approximately 13.5-18 kDa. The protein is expressed with a 6-amino acid His tag at the C-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
The product is a clear, colorless solution that has been sterilized by filtration.
Formulation
The CST3 protein solution is provided at a concentration of 1 mg/ml and is formulated in Phosphate Buffered Saline (pH 7.4) with 10% glycerol.
Stability
For short-term storage (up to 4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. Adding a carrier protein like HSA or BSA (0.1%) is advised for long-term storage. To maintain product integrity, avoid repeated freeze-thaw cycles.
Purity
The purity of CST3 is greater than 95.0% as determined by SDS-PAGE analysis.
Synonyms
Cystatin-C, Cystatin-3, Cst3.
Source
Sf9, Insect cells.
Amino Acid Sequence
ATPKQGPRML GAPEEADANE EGVRRALDFA VSEYNKGSND AYHSRAIQVV RARKQLVAGV NYFLDVEMGR TTCTKSQTNL TDCPFHDQPH LMRKALCSFQ IYSVPWKGTH SLTKFSCKNA HHHHHH.

Product Science Overview

Introduction

Cystatin-C is a member of the cystatin superfamily, which comprises proteins that function as cysteine protease inhibitors. These proteins play crucial roles in various physiological processes, including inflammation, tumor invasion, and metastasis. Cystatin-C is ubiquitously expressed in human tissues and body fluids, making it a significant biomarker for various diseases.

Structure and Function

Cystatin-C is a low molecular weight protein with a molecular mass of approximately 14.2 kDa . It is composed of 126 amino acids and is glycosylated. The protein inhibits several cysteine proteases, including papain and cathepsins B, H, K, L, and S . By inhibiting these proteases, Cystatin-C regulates proteolytic activities that are essential for maintaining cellular homeostasis.

Recombinant Production in sf9 Cells

The recombinant form of Cystatin-C (Mouse) is produced in Sf9 insect cells. Sf9 cells are derived from the fall armyworm, Spodoptera frugiperda, and are commonly used for the expression of recombinant proteins using the baculovirus expression system. This system is advantageous for producing high yields of recombinant proteins with post-translational modifications similar to those in mammalian cells.

The recombinant Cystatin-C produced in Sf9 cells is a single, glycosylated polypeptide chain containing amino acids 21-140 . The protein is purified to a high degree of purity (>95%) and is typically lyophilized for storage and transport .

Applications

Recombinant Cystatin-C is used in various research applications, including:

  • Biomarker Studies: Due to its ubiquitous presence and involvement in disease processes, Cystatin-C is studied as a biomarker for conditions such as chronic kidney disease, cardiovascular diseases, and neurological disorders.
  • Protease Inhibition Assays: Researchers use recombinant Cystatin-C to study the inhibition of cysteine proteases, which can provide insights into the regulation of proteolytic activities in different biological contexts.
  • Therapeutic Research: The protein’s role in inhibiting proteases makes it a potential candidate for therapeutic interventions in diseases characterized by excessive protease activity.

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THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
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