SDF 1b Feline

Stromal Cell-Derived Factor-1 beta Feline Recombinant (CXCL12)
Cat. No.
BT23622
Source
Escherichia Coli.
Synonyms
SDF-1, CXCL12, Pre-B cell growth-stimulating factor, PBSF, hIRH, chemokine (C-X-C motif) ligand 12, SDF1, SDF1B, TPAR1, SCYB12, SDF-1b, TLSF-b, 12-O-tetradecanoylphorbol 13-acetate repressed protein 1, Thymic lymphoma cell-stimulating factor, TLSF.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 97.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
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Description

Stromal Cell-Derived Factor-1 beta Feline Recombinant produced in E.Coli is a non-glycosylated, Polypeptide chain containing 72 amino acids and having a molecular mass of 8526 Dalton.
The SDF-1b is purified by proprietary chromatographic techniques.

Product Specs

Introduction
SDF-1, also known as stromal cell-derived factor-1, is a chemokine classified under the Chemokine (C-X-C motif) ligand 12 (CXCL12) designation. It exists in two isoforms, SDF-1α/CXCL12a and SDF-1β/CXCL12b, generated by alternative splicing of a single gene. Chemokines are characterized by the presence of four conserved cysteines forming two disulfide bonds. Belonging to the CXC chemokine group, CXCL12 proteins have a single amino acid separating their first cysteine pair. CXCL12 exhibits strong chemotactic activity towards lymphocytes and plays a crucial role in cell coordination during development. In embryogenesis, it guides hematopoietic cell migration from the fetal liver to the bone marrow. Mice with CXCL12 gene knockouts are not viable, dying before or within an hour of birth. Additionally, CXCL12a influences neuronal electrophysiology. CXCL12 expression is observed in various murine tissues, including the brain, thymus, heart, lung, liver, kidney, spleen, and bone marrow. CXCL12's receptor, CXCR4 (previously known as fusin), was once thought to exclusively interact with CXCL12. However, recent studies suggest that CXCL12 also binds to the CXCR7 receptor. The CXCL12 gene is located on human chromosome 10. High sequence identity is observed between human and mouse CXCL12 and CXCR4, with 99% and 90% similarity, respectively.
Description
Recombinant Feline Stromal Cell-Derived Factor-1 beta, produced in E. coli, is a non-glycosylated polypeptide chain comprising 72 amino acids. With a molecular weight of 8526 Daltons, SDF-1b undergoes purification using proprietary chromatographic techniques.
Physical Appearance
White, sterile-filtered lyophilized powder.
Formulation
The protein was freeze-dried from a sterile solution (1 mg/ml) without any additional additives.
Solubility
To reconstitute lyophilized SDF-1b, it is recommended to use sterile 18 MΩ-cm H2O at a concentration of at least 100 µg/ml. This solution can then be further diluted with other aqueous solutions.
Stability
Lyophilized SDF-1b remains stable at room temperature for up to 3 weeks. However, it is recommended to store it desiccated below -18°C. After reconstitution, CXCL12 should be stored at 4°C for 2-7 days. For long-term storage, it is recommended to freeze it below -18°C. To ensure optimal stability during long-term storage, consider adding a carrier protein like HSA or BSA (0.1%). Avoid repeated freeze-thaw cycles.
Purity
Exceeds 97.0% purity, as determined by RP-HPLC and SDS-PAGE analysis.
Biological Activity
The specific activity is determined by the ability of SDF-1b to chemoattract human peripheral T cells stimulated with IL-2 and PHA. This activity is observed within a concentration range of 10-75 ng/ml, corresponding to a specific activity of 13,334-100,000 IU/mg.
Synonyms
SDF-1, CXCL12, Pre-B cell growth-stimulating factor, PBSF, hIRH, chemokine (C-X-C motif) ligand 12, SDF1, SDF1B, TPAR1, SCYB12, SDF-1b, TLSF-b, 12-O-tetradecanoylphorbol 13-acetate repressed protein 1, Thymic lymphoma cell-stimulating factor, TLSF.
Source
Escherichia Coli.
Amino Acid Sequence
KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNKRF KM.

Product Science Overview

Introduction

Stromal Cell-Derived Factor-1 beta (SDF-1β), also known as CXCL12, is a chemokine that plays a crucial role in various physiological and pathological processes. It is a member of the CXC chemokine family and is known for its ability to attract immune cells to sites of inflammation or injury. The feline recombinant form of this protein is used in research to study its effects in various biological contexts.

Structure and Function

CXCL12 is produced as a result of alternative splicing of the CXCL12 gene, resulting in two main isoforms: SDF-1α and SDF-1β. The beta isoform, SDF-1β, is composed of 72 amino acids and has a molecular weight of approximately 8.5 kDa . This chemokine is known for its chemoattractant properties, particularly on T-lymphocytes and monocytes, but not on neutrophils . It activates the C-X-C chemokine receptor CXCR4, leading to a rapid and transient increase in intracellular calcium ions and chemotaxis .

Biological Activity

SDF-1β is involved in various biological processes, including:

  • Immune Response: It recruits activated leukocytes to sites of inflammation, playing a critical role in the immune response .
  • Hematopoiesis: It is essential for the migration and homing of hematopoietic stem cells to the bone marrow.
  • Angiogenesis: It promotes the formation of new blood vessels, which is vital for tissue repair and regeneration.
  • Cancer: It has been implicated in tumor growth and metastasis by promoting the migration of cancer cells to distant sites.
Recombinant Feline CXCL12

The recombinant form of feline CXCL12 is produced using E. coli expression systems. This recombinant protein is typically used in research to study its effects in various biological assays. It is available in both carrier-free and carrier-containing formulations, with the carrier protein often being Bovine Serum Albumin (BSA) to enhance stability and shelf-life .

Applications in Research

Recombinant feline CXCL12 is used in a variety of research applications, including:

  • Cell Migration Assays: To study the chemotactic properties of CXCL12 on different cell types.
  • Receptor Binding Studies: To investigate the interaction between CXCL12 and its receptor CXCR4.
  • Cancer Research: To explore the role of CXCL12 in tumor growth and metastasis.
  • Stem Cell Research: To understand the mechanisms of stem cell homing and migration.

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