CTLA4 Human, igG-His

Cytotoxic T-Lymphocyte Associated Antigen-4 Human Recombinant, igG-His Tag
Cat. No.
BT20411
Source
Sf9, Baculovirus cells.
Synonyms
CTLA4, ALPS5, CD, CD152, CELIAC3, CTLA-4, GRD4, GSE, IDDM12, CD152, Cytotoxic T-Lymphocyte Associated Antigen-4, igG-His Tag.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 95% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

CTLA4 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 368 amino acids (36-161a.a.) and having a molecular mass of 40.8kDa (Molecular size on SDS-PAGE will appear at approximately 40-57kDa).
CTLA4 is expressed with a 239 amino acid hIgG-His-tag at C-Terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
CTLA-4, a protein belonging to the immunoglobulin superfamily, is responsible for transmitting inhibitory signals to T cells. This protein comprises a V domain, a transmembrane domain, and a cytoplasmic tail. Notably, alternate transcriptional splice variants give rise to different isoforms of CTLA-4. While the membrane-bound isoform exists as a homodimer interconnected by a disulfide bond, the soluble isoform functions as a monomer. Mutations in the gene encoding CTLA-4 have been implicated in a range of autoimmune diseases including insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, and thyroid-associated orbitopathy.
Description
Produced in Sf9 Baculovirus cells, CTLA4 is a single, glycosylated polypeptide chain with a molecular weight of 40.8kDa (368 amino acids, 36-161a.a.). However, on SDS-PAGE, the molecular size appears to be between 40-57kDa. This CTLA4 protein is expressed with a 239 amino acid hIgG-His-tag at the C-Terminus and purified using proprietary chromatographic techniques.
Physical Appearance
A sterile, colorless solution.
Formulation
The CTLA4 protein solution (0.5mg/ml) is formulated in Phosphate Buffered Saline (pH 7.4) and contains 10% glycerol.
Stability
For short-term storage (2-4 weeks), the CTLA4 protein solution should be kept at 4°C. For longer periods, storage at -20°C is recommended. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. It's crucial to avoid repeated freeze-thaw cycles.
Purity
The purity of the CTLA4 protein is greater than 95% as determined by SDS-PAGE analysis.
Synonyms
CTLA4, ALPS5, CD, CD152, CELIAC3, CTLA-4, GRD4, GSE, IDDM12, CD152, Cytotoxic T-Lymphocyte Associated Antigen-4, igG-His Tag.
Source
Sf9, Baculovirus cells.
Amino Acid Sequence
ADLKAMHVAQ PAVVLASSRG IASFVCEYAS PGKATEVRVT VLRQADSQVT EVCAATYMMG NELTFLDDSI CTGTSSGNQV NLTIQGLRAM DTGLYICKVE LMYPPPYYLG IGNGTQIYVI DPEPCPDSDL EPKSCDKTHT CPPCPAPELL GGPSVFLFPP KPKDTLMISR TPEVTCVVVD VSHEDPEVKF NWYVDGVEVH NAKTKPREEQ YNSTYRVVSV LTVLHQDWLN GKEYKCKVSN KALPAPIEKT ISKAKGQPRE PQVYTLPPSR DELTKNQVSL TCLVKGFYPS DIAVEWESNG QPENNYKTTP PVLDSDGSFF LYSKLTVDKS RWQQGNVFSC SVMHEALHNH YTQKSLSLSP GKHHHHHH.

Product Science Overview

Introduction

Cytotoxic T-Lymphocyte Associated Antigen-4 (CTLA-4), also known as CD152, is a protein receptor that plays a crucial role in the regulation of the immune system. It is a member of the immunoglobulin superfamily and functions as an immune checkpoint, downregulating immune responses . The human recombinant form of CTLA-4, tagged with an immunoglobulin G (IgG) and a histidine (His) tag, is used in various research and therapeutic applications.

Structure and Function

CTLA-4 is expressed primarily on the surface of activated T cells and regulatory T cells (Tregs). It competes with the costimulatory receptor CD28 for binding to B7 molecules (CD80 and CD86) on antigen-presenting cells (APCs). Unlike CD28, which provides a stimulatory signal, CTLA-4 delivers an inhibitory signal that attenuates T cell activation .

Role in Immune Regulation

The primary function of CTLA-4 is to maintain immune homeostasis and prevent autoimmunity. By inhibiting T cell activation, CTLA-4 ensures that the immune response is proportional to the threat and does not damage normal tissues . This regulatory mechanism is essential for preventing autoimmune diseases and maintaining tolerance to self-antigens.

Therapeutic Applications

CTLA-4 has become a significant target in cancer immunotherapy. Monoclonal antibodies that block CTLA-4, such as ipilimumab, have been developed to enhance the immune response against tumors. By inhibiting CTLA-4, these therapies aim to boost T cell activity and promote the destruction of cancer cells . Clinical trials have shown promising results, particularly in the treatment of metastatic melanoma .

Human Recombinant CTLA-4 (IgG-His Tag)

The human recombinant form of CTLA-4, tagged with IgG and His, is produced using recombinant DNA technology. This form is used in research to study the protein’s structure, function, and interactions. The IgG tag facilitates purification and detection, while the His tag allows for efficient purification using metal affinity chromatography .

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