CTF1 Human

CT-1 is a 201 amino acid protein with a molecular mass of approximately 21 kDa . The recombinant form of CT-1 is typically produced in Escherichia coli (E. coli) and is available in both glycosylated and non-glycosylated forms . The protein is often lyophilized and can be reconstituted in sterile solutions for various applications .

CT-1 is a pleiotropic cytokine, meaning it has multiple effects on different cell types . It activates gp130-dependent signaling and stimulates the Janus kinase/signal transducers and activators of transcription (JAK/STAT) pathway . This signaling pathway is crucial for transducing hypertrophic and cytoprotective signals in cardiac myocytes . Additionally, CT-1 has neurotrophic functions and is involved in the survival of motoneurons .

CT-1 expression is augmented after hypoxic stimulation and can protect cardiac cells during ischemic events . It has been shown to induce the expression of protective heat shock proteins (hsps) in cardiac cells . Elevated levels of CT-1 have been observed in patients with heart failure, dilatative cardiomyopathy, mitral regurgitation, stable and unstable angina, and after acute myocardial infarction .

Recombinant human CT-1 is used in various research applications, including:

• Cell Proliferation Assays: CT-1 is measured in cell proliferation assays using TF-1 human erythroleukemic cells .
• Cardiac Research: Due to its role in inducing hypertrophic responses and protecting cardiac cells, CT-1 is extensively studied in cardiac research .
• Neurobiology: CT-1’s neurotrophic functions make it a subject of interest in neurobiology research .

Recombinant CT-1 is typically stored at -20 to -70°C and should be reconstituted in sterile solutions containing human or bovine serum albumin to enhance stability . It is important to avoid repeated freeze-thaw cycles to maintain the protein’s integrity .