CTF1 Human, His
Cardiotrophin-1 Human Recombinant, His Tag
Cat. No.
BT19889
Source
Escherichia Coli.
Synonyms
CTF1, CT1, CT-1, Cardiophin 1.
Appearance
Purity
Purity of CTF1 Human Recombinant is greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
The Cardiotrophin His-Tagged Fusion Protein Human, produced in E. coli, is 22.5 kDa protein containing 200 amino acid residues of the human Cardiotrophin and 12 additional amino acid residues – His Tag (underlined).
Product Specs
Introduction
Cardiotrophin 1 (CT-1), a member of the interleukin-6 superfamily, is a 201 amino acid protein known for its ability to induce hypertrophy in cardiac myocytes. Found in both cardiac myocytes and nonmyocytes, CT-1 mRNA is also abundant in adult human lungs and fetal and adult airway smooth muscle cells. CT-1 activates gp130 dependent signaling and the Janus kinase/signal transducers and activators of transcription (JAK/STAT) pathway, transducing hypertrophic and cytoprotective signals in cardiac myocytes. Furthermore, CT-1 exhibits neurotrophic properties, with deficiencies leading to increased motoneuron cell death in mice spinal cord and brainstem nuclei during early development. Acting as a hepatocyte survival factor, CT-1 can reduce hepatocellular damage in acute liver injury animal models. Hypoxic stimulation increases CT-1 expression, offering protection to cardiac cells when administered before simulated ischemia or during reoxygenation. This protective effect extends to inducing the expression of heat shock proteins (hsps) in cardiac cells. CT-1 also elevates ventricular expression of ANP, brain natriuretic peptide (BNP), and angiotensinogen mRNA. Notably, elevated Cardiophin 1 levels are observed in patients with heart failure, dilatative cardiomyopathy, mitral regurgitation, angina, and after acute myocardial infarction.
Description
Produced in E. coli, the Cardiotrophin His-Tagged Fusion Protein Human is a 22.5 kDa protein. It consists of 200 amino acid residues from human Cardiotrophin and a 12 amino acid residue His Tag (HHHHHH).
Formulation
CTF1 was lyophilized from a 0.5 mg/ml solution in 0.05M Acetate buffer with a pH of 4. The solution was filtered through a 0.4 μm filter before lyophilization.
Solubility
To prepare a working stock solution of approximately 0.5 mg/ml, add 0.1M Acetate buffer (pH 4) to the lyophilized pellet and allow it to dissolve completely. For use at a higher pH, dilute the stock solution to 10 µg/ml with the appropriate buffer. The solubility of this antigen is limited at higher concentrations. This product is not sterile; filter it through a sterile filter before use in cell cultures.
Stability
Store the lyophilized protein at -20°C. After reconstitution, aliquot the product to minimize freeze-thaw cycles. The reconstituted protein remains stable at 4°C for a limited time, showing no changes for up to two weeks.
Purity
SDS-PAGE analysis indicates that the purity of CTF1 Human Recombinant is greater than 90%.
Synonyms
CTF1, CT1, CT-1, Cardiophin 1.
Source
Escherichia Coli.
Amino Acid Sequence
MRGSHHHHHH GSSRREGSLE DPQTDSSVSL LPHLEAKIRQ THSLAHLLTK YAEQLLQEYV QLQGDPFGLPSFSPPRLPVA GLSAPAPSHA GLPVHERLRL DAAALAALPP LLDAVCRRQA ELNPRAPRLL RRLEDAARQA RALGAAVEAL LAALGAANRG PRAEPPAATA SAASATGVFP AKVLGLRVCG LYREWLSRTE GDLGQLLPGG SA.
Product Science Overview
Molecular Characteristics
- Amino Acid Sequence: Cardiotrophin-1 consists of 201 amino acids. The recombinant version, often produced in human 293 cells (HEK293), includes a polyhistidine tag (His Tag) at the N-terminus .
- Molecular Weight: The protein has a calculated molecular weight of approximately 23 kDa. However, due to glycosylation, it migrates as 27-30 kDa on SDS-PAGE .
- Purity: The recombinant protein is typically purified to greater than 90% purity as determined by SDS-PAGE and SEC-MALS .
Biological Functions
Cardiotrophin-1 is known for its ability to:
- Induce Cardiac Hypertrophy: It stimulates the hypertrophic growth of cardiac myocytes, which is a critical response during heart development and in certain pathological conditions .
- Activate Signaling Pathways: CT-1 binds to and activates the ILST/gp130 receptor, which in turn activates the Janus kinase/signal transducers and activators of transcription (JAK/STAT) pathway. This signaling cascade is essential for transducing hypertrophic and cytoprotective signals in cardiac cells .
- Neurotrophic Functions: CT-1 also has neurotrophic properties, supporting the survival of motoneurons in the spinal cord and brainstem .
- Hepatocyte Survival: It acts as a hepatocyte survival factor, reducing liver cell damage in models of acute liver injury .
Clinical Relevance
Recombinant Production
- Expression System: The recombinant form of Cardiotrophin-1 is often produced in Escherichia coli or human 293 cells (HEK293) .
- Formulation: The protein is typically lyophilized from a filtered solution and can be reconstituted in sterile water or other suitable buffers .
- Storage: For long-term storage, the lyophilized protein should be kept at -20°C or lower. Once reconstituted, it should be stored at 4°C for short-term use and at -70°C for long-term storage .
Cardiotrophin-1 (Human Recombinant, His Tag) is a valuable tool in research due to its significant roles in cardiac hypertrophy, neuroprotection, and hepatocyte survival. Its recombinant form allows for detailed studies and potential therapeutic applications.
Quick Inquiry
Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *
