CSNK2A1 Human
Casein Kinase 2 alpha 1 Human Recombinant
Cat. No.
BT30061
Source
Escherichia Coli.
Synonyms
Casein kinase II subunit alpha, EC 2.7.11.1, CK II, CK2A1, CKII alpha, CSNK2A1, PKCK2, CSK21.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 99% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
Casein Kinase 2 alpha 1 Human Recombinant is a non-glycosylated polypeptide having a molecular mass of 45.1 kDa.
Casein Kinase 2 is purified by proprietary chromatographic techniques.
Casein Kinase 2 is purified by proprietary chromatographic techniques.
Product Specs
Introduction
Casein kinase 2 (EC 2.7.11.1) is a serine/threonine-selective protein kinase essential for various cellular processes, including cell cycle regulation, DNA repair, and circadian rhythm control. This kinase comprises two alpha and two beta subunits, with the alpha subunits housing the catalytic kinase domain. Casein kinase 2's activity is heightened following the activation of the Wnt signaling pathway. This activation process involves a Pertussis toxin-sensitive G protein and Disheveled, acting as intermediaries between the Wnt-activated Frizzled receptor and casein kinase 2. Notably, mice lacking the casein kinase 2 alpha prime exhibit developmental defects in sperm morphology.
Description
Recombinant human Casein Kinase 2 alpha 1 is a non-glycosylated polypeptide with a molecular weight of 45.1 kDa. It is purified using proprietary chromatographic methods and is free of detectable protease activity.
Physical Appearance
A clear solution that has been sterilized by filtration.
Formulation
CSNK2A1 is supplied in a buffer of 25mM Tris-HCl, 500mM NaCl, 1mM DTT, 500µM PMSF, and 50% glycerol at pH 8.5.
Stability
For short-term storage (2-4 weeks), the product should be kept at 4°C. For extended storage, it is recommended to store the product frozen at -20°C. Repeated freezing and thawing should be avoided.
Unit Definition
One unit (U) of activity is defined as the amount of enzyme required to catalyze the transfer of 1 micromole of phosphate per minute to the synthetic peptide substrate RRRDDDSDDD at 37 degrees Celsius. The specific activity of this product is greater than 1,320 U/mg.
Purity
The purity of this product is determined by SDS-PAGE analysis and is greater than 99%.
Synonyms
Casein kinase II subunit alpha, EC 2.7.11.1, CK II, CK2A1, CKII alpha, CSNK2A1, PKCK2, CSK21.
Source
Escherichia Coli.
Product Science Overview
Structure and Composition
Function
CK2α is involved in the phosphorylation of acidic proteins such as casein. It regulates numerous cellular processes, including:
- Cell Cycle Control: CK2α plays a role in cell cycle progression by phosphorylating various substrates involved in cell division .
- Apoptosis: It is implicated in the regulation of programmed cell death, ensuring proper cellular turnover and response to stress .
- Circadian Rhythm: CK2α is involved in the regulation of the body’s internal clock, affecting sleep-wake cycles and other rhythmic processes .
- DNA Damage Response: CK2α targets proteins such as XRCC1 and BRCA1, which are involved in the repair of DNA damage caused by UV irradiation .
Clinical Significance
Mutations in the CSNK2A1 gene have been associated with various diseases, including:
Research and Applications
Recombinant human CK2α is widely used in research to study its role in cellular processes and disease mechanisms. It serves as a valuable tool for understanding the molecular pathways regulated by CK2α and for developing potential therapeutic interventions.
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