CSNK2A1 Human, His
Casein Kinase 2 alpha 1 Human Recombinant, His
Cat. No.
BT30125
Source
Escherichia Coli.
Synonyms
Casein kinase II subunit alpha, EC 2.7.11.1, CK II, CK2A1, CKII alpha, CSNK2A1, PKCK2, CSK21.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
CK2A Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain encoding the sequence of 411 amino acids and having a molecular mass of 47.3 kDa.
Casein Kinase 2 alpha subunit is purified by proprietary chromatographic techniques.
Casein Kinase 2 alpha subunit is purified by proprietary chromatographic techniques.
Product Specs
Introduction
Casein kinase 2 (EC2.7.11.1) is a serine/threonine-selective protein kinase that functions as a tetramer composed of two alpha subunits and two beta subunits. The alpha subunits contain the catalytic kinase domain. This kinase plays a role in various cellular processes, including cell cycle control, DNA repair, and the regulation of the circadian rhythm. Casein kinase 2 activity is known to be activated following the activation of the Wnt signaling pathway. A Pertussis toxin-sensitive G protein and Disheveled appear to mediate the connection between Wnt-mediated activation of the Frizzled receptor and the activation of casein kinase 2. Mice lacking casein kinase 2 alpha prime exhibit defects in the morphology of developing sperm.
Description
Recombinant human CSNK2A1, expressed in E. coli, is a single, non-glycosylated polypeptide chain containing 411 amino acids. It has a molecular weight of 47.3 kDa. This protein corresponds to the alpha subunit of Casein Kinase 2 and is purified using proprietary chromatographic techniques.
Physical Appearance
Clear, sterile-filtered solution.
Formulation
CSNK2A1 is supplied in a buffer consisting of 20mM Tris pH 8.0, 500mM NaCl, 1mM DTT, and 50% glycerol.
Stability
For short-term storage (2-4 weeks), keep at 4°C. For long-term storage, freeze at -20°C. Avoid repeated freeze-thaw cycles.
Purity
Purity is greater than 90% as determined by SDS-PAGE analysis.
Synonyms
Casein kinase II subunit alpha, EC 2.7.11.1, CK II, CK2A1, CKII alpha, CSNK2A1, PKCK2, CSK21.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MSGPVPSRAR VYTDVNTHRP REYWDYESHV VEWGNQDDYQLVRKLGRGKY SEVFEAINIT NNEKVVVKIL KPVKKKKIKR EIKILENLRG GPNIITLADI VKDPVSRTPA LVFEHVNNTD FKQLYQTLTD YDIRFYMYEI LKALDYCHSM GIMHRDVKPH NVMIDHEHRK LRLIDWGLAE FYHPGQEYNV RVASRYFKGP ELLVDYQMYD YSLDMWSLGC MLASMIFRKE PFFHGHDNYD QLVRIAKVLG TEDLYDYIDK YNIELDPRFN DILGRHSRKRWERFVHSENQ HLVSPEALDF LDKLLRYDHQ SRLTAREAME HPYFYTVVKD QARMGSSSMP GGSTPVSSAN MMSGISSVPT PSPLGPLAGS PVIAAANPLG MPVPAAAGAQ Q.
Product Science Overview
Structure and Composition
The CK2 complex is a tetramer composed of two catalytic alpha subunits (CSNK2A1 and CSNK2A2) and two regulatory beta subunits (CSNK2B). The alpha subunits are responsible for the catalytic activity, while the beta subunits undergo autophosphorylation . The human recombinant form of CSNK2A1 is often tagged with a His-tag to facilitate purification and detection in research applications .
Function and Significance
CSNK2A1 is involved in numerous cellular processes, including:
- Cell Cycle Control: It regulates the progression of the cell cycle by phosphorylating various substrates involved in cell division .
- Apoptosis: CSNK2A1 plays a role in programmed cell death, influencing the balance between cell survival and apoptosis .
- Circadian Rhythm: It is implicated in the regulation of circadian rhythms, affecting the body’s internal clock .
- Transcription and Gene Expression: CSNK2A1 is involved in the regulation of transcription and gene expression by phosphorylating transcription factors and other proteins involved in these processes .
Clinical Relevance
Research Applications
The human recombinant form of CSNK2A1, tagged with a His-tag, is widely used in research to study its function and interactions. This recombinant protein is produced in various expression systems, such as baculovirus or E. coli, and is purified using chromatographic techniques . It is used in kinase assays, structural studies, and drug discovery efforts to develop inhibitors targeting CK2 activity .
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