CK2a Zea Mays

The nucleotide sequence of the cDNA coding for the alpha subunit of Casein Kinase 2 from Zea mays has been determined . The cDNA clone contains an open reading frame of 996 nucleotides, encoding a polypeptide comprising 332 amino acids . This sequence information has been pivotal in producing the recombinant form of the enzyme.

The recombinant Casein Kinase 2 alpha from Zea mays is typically produced in Escherichia coli (E. coli) expression systems . The enzyme is a non-glycosylated polypeptide with a molecular mass of approximately 39.2 kDa . It is purified using proprietary chromatographic techniques to achieve a purity greater than 99%, as determined by SDS-PAGE .

Recombinant Casein Kinase 2 alpha from Zea mays is used extensively in laboratory research. It is ideal for immunization protocols to generate antibodies that specifically target the protein of interest . The enzyme’s activity is measured using synthetic peptides, and it is supplied in formulations that ensure stability and activity over extended periods .

The recombinant enzyme is typically stored at 4°C for short-term use and at -20°C for long-term storage . It is important to avoid multiple freeze-thaw cycles to maintain the enzyme’s activity and stability .