CSNK2A1 Antibody

CK2 is ubiquitously expressed and constitutively active, meaning it does not require external signals to be activated. The enzyme is involved in the phosphorylation of over 300 substrates, which include proteins involved in DNA repair, cell cycle regulation, and apoptosis . Some of the key substrates of CK2 include:

• XRCC1: Involved in DNA repair.
• BRCA1: A tumor suppressor protein.
• p53: Another tumor suppressor protein that regulates the cell cycle and prevents cancer formation .

CK2α specifically has been implicated in the regulation of the NF-κB pathway, which is crucial for immune response and inflammation. UV irradiation can stimulate CK2-mediated phosphorylation of IκBα, leading to its degradation and the subsequent activation of NF-κB .

CK2α is dysregulated in various cancers and neurodegenerative diseases such as Alzheimer’s and Parkinson’s diseases. Its overexpression has been observed in several types of cancer, including chronic lymphocytic leukemia, chronic myeloid leukemia, acute lymphoblastic leukemia, acute myeloid leukemia, and multiple myeloma . Due to its role in these diseases, CK2α is considered a potential therapeutic target.

The Mouse Anti-Human CK2α Antibody is a monoclonal antibody used in various research applications to detect CK2α in human samples. This antibody is produced by immunizing mice with a synthetic peptide corresponding to amino acid residues near the carboxy-terminus of human CK2α. The antibody is then purified using protein A and peptide affinity chromatography .

Applications

The Mouse Anti-Human CK2α Antibody is commonly used in:

• Western Blotting: To detect CK2α in cell lysates.
• Immunohistochemistry: To study the localization of CK2α in tissue samples.
• Immunocytochemistry: To visualize CK2α in cultured cells .

Storage and Handling

The antibody is typically supplied in a buffer containing sodium HEPES, sodium chloride, bovine serum albumin, and glycerol. It should be stored at -20°C and should not be aliquoted to maintain its stability .