CoV Spike Porcine, PTT1E11
Description
Product Specs
The antibody was developed using purified porcine coronavirus clone PTT1E11. (eptopoe not mapped)
IgG2a
Product Science Overview
The Mouse Anti Porcine Coronavirus Spike Monoclonal Antibody, clone PTT1E11, is a specialized antibody developed for research and diagnostic purposes. This monoclonal antibody specifically targets the spike protein of porcine coronaviruses, which are significant pathogens affecting swine populations globally. The spike protein is a critical component of the coronavirus structure, responsible for virus entry into host cells.
Monoclonal antibodies are produced using hybridoma technology, which involves the fusion of an antibody-producing B cell with a myeloma (cancer) cell. This fusion results in a hybrid cell, or hybridoma, that can produce large quantities of a single type of antibody. The development of clone PTT1E11 involves the following steps:
- Immunization: Mice are immunized with the spike protein of the porcine coronavirus. This process stimulates the mouse’s immune system to produce antibodies against the spike protein.
- Cell Fusion: Spleen cells from the immunized mice, which produce the desired antibodies, are fused with myeloma cells. This fusion creates hybridoma cells capable of continuous growth and antibody production.
- Screening and Selection: The hybridoma cells are screened to identify those producing the desired antibody. Clone PTT1E11 is selected for its high specificity and affinity for the porcine coronavirus spike protein.
- Production and Purification: The selected hybridoma cells are cultured, and the monoclonal antibodies are harvested from the culture medium. The antibodies are then purified to ensure high purity and concentration.
The Mouse Anti Porcine Coronavirus Spike Monoclonal Antibody, clone PTT1E11, has several important applications in research and diagnostics:
- Virus Detection: This antibody can be used in various immunoassays, such as ELISA (enzyme-linked immunosorbent assay) and Western blotting, to detect the presence of porcine coronavirus in samples.
- Vaccine Development: Researchers use this antibody to study the immune response to porcine coronavirus and to evaluate the efficacy of potential vaccines.
- Pathogenesis Studies: The antibody helps in understanding the mechanisms of virus entry and infection, which is crucial for developing antiviral strategies.
- Therapeutic Research: Clone PTT1E11 can be used to explore potential therapeutic interventions targeting the spike protein of porcine coronaviruses.
