CoV Spike Bovine
Description
Product Specs
The product is supplied as a 1 milligram per milliliter solution in 1X phosphate-buffered saline (PBS) at a pH of 7.2 and contains 0.01% sodium azide (NaN3) as a preservative.
For short-term storage, the product should be kept at a temperature of 4 degrees Celsius, where it remains stable for up to two weeks. For long-term storage, it is recommended to store the product at -20 degrees Celsius to ensure its stability.
The antibody was developed using purified bovine coronavirus. (eptopoe not mapped)
IgG1
Product Science Overview
The Mouse Anti Bovine Coronavirus Spike Monoclonal Antibody is a specialized antibody designed to target the spike protein of the bovine coronavirus (BCoV). This antibody is produced using mouse cells and is monoclonal, meaning it is derived from a single clone of cells and recognizes a specific epitope on the spike protein.
Bovine coronavirus is a significant pathogen in cattle, causing severe diarrhoea in newborn calves, which can lead to high mortality rates. It is also associated with winter dysentery in adult cattle and respiratory infections in both calves and adult cattle . The virus belongs to the Coronaviridae family and is similar in structure to other coronaviruses, including the human SARS-CoV and MERS-CoV.
The spike protein, also known as the surface antigen or peplomer, is a crucial component of the bovine coronavirus. It plays a fundamental role in the virus’s ability to attach to and enter host cells. The spike protein is a glycoprotein that protrudes from the viral envelope, facilitating the initial interaction with the host cell receptor .
Monoclonal antibodies are antibodies that are made by identical immune cells, all of which are clones of a unique parent cell. They have monovalent affinity, binding to the same epitope. The production of monoclonal antibodies involves the fusion of spleen cells from an immunized mouse with myeloma cells, creating a hybridoma that can be cultured to produce large quantities of the antibody .
The production process begins with the immunization of mice with the bovine coronavirus. The spleen cells from these immunized mice are then fused with myeloma cells to create hybridomas. These hybridomas are screened for the production of the desired antibody, which is then purified using affinity chromatography .
The Mouse Anti Bovine Coronavirus Spike Monoclonal Antibody is used in various research applications, including:
- Virus Detection: It can be used in immunoassays to detect the presence of bovine coronavirus in samples.
- Vaccine Development: It aids in the study of the immune response to the spike protein, which is essential for developing effective vaccines.
- Pathogenesis Studies: It helps in understanding the mechanisms of viral entry and infection in host cells.
