CoV2 Nucleocapsid Polyclonal

The N protein consists of two main domains:

1. N-terminal domain (NTD)
2. C-terminal domain (CTD)

These domains are flanked by three disorganized regions, which contribute to the protein’s flexibility and its ability to bind RNA . The N protein’s strong immunogenicity makes it a valuable target for antibody production, as it can elicit a robust immune response.

Polyclonal antibodies are a mixture of antibodies produced by different B cell clones in the body. They recognize multiple epitopes on the same antigen, providing a broader immune response. The Full Length CoV2 Nucleocapsid Polyclonal Antibody is generated by immunizing animals, typically rabbits, with the full-length recombinant SARS-CoV-2 nucleocapsid protein . This process results in the production of antibodies that can bind to various epitopes on the N protein.

The Full Length CoV2 Nucleocapsid Polyclonal Antibody has several applications in research and diagnostics:

• Western Blot (WB): Used to detect the presence of the N protein in a sample.
• Immunohistochemistry (IHC): Allows for the visualization of the N protein in tissue samples.
• Immunocytochemistry (ICC/IF): Used to detect the N protein in cultured cells.
• Flow Cytometry (Flow): Enables the analysis of the N protein in cell populations.
• Enzyme-Linked Immunosorbent Assay (ELISA): Quantifies the N protein in various samples.
• Immunoprecipitation (IP): Isolates the N protein from a mixture of proteins .

The antibody is typically stored as a concentrated solution at -20°C to maintain its stability. For short-term storage (1-2 weeks), it can be kept at 4°C. It is important to avoid multiple freeze-thaw cycles to preserve the antibody’s integrity .