Greater than 90% as determined by SDS-PAGE gel.
SARS Nucleoprotein Biotinylated antibody clone PT3851 is specific for the nucleocapsid SARS-CoV protein and recognizes the nucleoprotein of SARS-CoV-2 by ELISA.
The SARS Nucleoprotein Biotinylated antibody, specifically clone PT3851, demonstrates specificity for the nucleocapsid protein of SARS-CoV. Furthermore, it exhibits recognition of the SARS-CoV-2 nucleoprotein in ELISA assays.
The antibody is provided at a concentration of 1 mg/ml and is formulated in a solution of Dulbecco's Phosphate-Buffered Saline (DPBS) containing 1% Bovine Serum Albumin (BSA) and 0.1% Proclin 950 as a preservative.
This antibody is suitable for use in Enzyme-Linked Immunosorbent Assay (ELISA) applications.
The purity of this antibody is greater than 90%, as determined by SDS-PAGE gel analysis.
For short-term storage, the antibody should be stored at 4°C and is stable for up to 2 weeks. For long-term storage, it is recommended to store the antibody at -20°C to ensure its stability.
Protein A affinity purified.
PT3851
Recombinant protein fragment 1-49 a.a. of the SARS nucleoprotein.
Mouse IgG2b.
The Mouse Anti-SARS Nucleocapsid Biotinylated antibody is a monoclonal antibody specifically designed to target the nucleocapsid protein of the Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV) and its successor, SARS-CoV-2, which is responsible for the COVID-19 pandemic. This antibody is biotinylated, meaning it has been chemically linked to biotin, a vitamin that allows for easy detection and purification in various laboratory assays.
The nucleocapsid (N) protein is one of the structural proteins of coronaviruses, including SARS-CoV and SARS-CoV-2. It plays a crucial role in the viral replication cycle by binding to the viral RNA genome and packaging it into a ribonucleoprotein complex. The N protein is highly immunogenic, making it an excellent target for diagnostic assays and therapeutic interventions .
Monoclonal antibodies are antibodies that are derived from a single clone of cells and are therefore identical in structure. They are highly specific to a particular antigen, in this case, the SARS-CoV-2 nucleocapsid protein. The production of monoclonal antibodies involves the fusion of an antibody-producing B cell with a myeloma cell, creating a hybridoma that can be cultured to produce large quantities of the antibody .
Biotinylation is the process of attaching biotin to proteins and other macromolecules. Biotin has a strong affinity for streptavidin and avidin, proteins that are commonly used in laboratory assays. This strong binding allows for the easy capture and detection of biotinylated molecules. In the context of the Mouse Anti-SARS Nucleocapsid Biotinylated antibody, biotinylation facilitates its use in various immunoassays, such as ELISA (Enzyme-Linked Immunosorbent Assay) and Western blotting .
The Mouse Anti-SARS Nucleocapsid Biotinylated antibody is used in several applications: