Greater than 90% as determined by SDS-PAGE gel.
SARS Nucleoprotein HRP antibody clone PT3851 is specific for the nucleocapsid SARS-CoV protein and recognizes the nucleoprotein of SARS-CoV-2 by ELISA.
Protein A affinity purified.
PT3851
Recombinant protein fragment 1-49 a.a. of the SARS nucleoprotein.
Mouse IgG2b.
The severe acute respiratory syndrome coronavirus (SARS-CoV) and its successor, SARS-CoV-2, have posed significant global health challenges. One of the critical components in the study and detection of these viruses is the nucleocapsid (N) protein. This protein is highly immunogenic and plays a crucial role in the virus’s life cycle, making it a prime target for diagnostic and therapeutic research.
The N protein of SARS-CoV and SARS-CoV-2 is one of the four main structural proteins, alongside the spike (S), membrane (M), and envelope (E) proteins . It is the most abundant protein within the virion and is essential for RNA packaging within the ribonucleoprotein complex . The N protein also participates in RNA replication and transcription, virion assembly, and evasion of the host immune response .
Structurally, the N protein consists of 419 amino acids and is divided into several regions: the N-arm, RNA-binding domain, oligomerization domain, and C-tail . These regions are linked by a central Ser/Arg-rich flexible linker region . The high immunogenicity of the N protein makes it an excellent candidate for generating antibodies.
Mouse anti-SARS nucleocapsid antibodies are generated by immunizing mice with the recombinant N protein. These antibodies are highly specific and can recognize both linear and conformational epitopes of the N protein . The production of these antibodies involves several steps, including the administration of the recombinant N protein to mice, followed by the collection and purification of the antibodies from the serum.
Horseradish peroxidase (HRP) is an enzyme commonly used in biochemistry applications, particularly in enzyme-linked immunosorbent assays (ELISA) and western blotting. Conjugating HRP to mouse anti-SARS nucleocapsid antibodies enhances their detection capabilities. The HRP enzyme catalyzes the oxidation of substrates, producing a detectable signal, usually a color change, which indicates the presence of the target antigen.
The mouse anti-SARS nucleocapsid HRP conjugate is widely used in various diagnostic assays to detect the presence of SARS-CoV and SARS-CoV-2. These assays are crucial for diagnosing infections, monitoring disease progression, and conducting epidemiological studies. The high specificity and sensitivity of these conjugates make them invaluable tools in both research and clinical settings .