COPS8 Human
Description
COPS8 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
MVLPRKPVAG ALDVSFNKFI PLSEPAPVPP IPNEQQLARL TDYVAFLEN.
Product Science Overview
COPS8, also known as CSN8, is one of the eight subunits of the CSN complex. It is a single, non-glycosylated polypeptide chain containing 229 amino acids and has a molecular mass of approximately 25.3 kDa . The protein is fused to a 20 amino acid His-tag at the N-terminus, which aids in its purification through chromatographic techniques .
The CSN complex, including COPS8, is involved in the deneddylation of cullin-RING ligases (CRLs), which are essential for the ubiquitination and subsequent proteasomal degradation of target proteins. This process is vital for the regulation of various cellular functions, including cell cycle progression, signal transduction, and transcriptional regulation.
The COP9 signalosome complex, with COPS8 as a critical component, plays a significant role in several biological processes:
- Protein Degradation: By regulating the activity of CRLs, the CSN complex controls the degradation of numerous proteins, ensuring proper cellular function and response to environmental changes.
- Developmental Processes: The CSN complex is involved in the regulation of photomorphogenesis in plants, a process by which plants develop in response to light. Although this function is more prominent in plants, the conservation of the CSN complex across species suggests its importance in other organisms, including humans.
- Cell Cycle Regulation: The CSN complex influences the cell cycle by modulating the stability of key regulatory proteins, thereby ensuring proper cell division and proliferation.
Recombinant human COPS8 is produced in Escherichia coli (E. coli) as a single polypeptide chain. The recombinant protein is purified using proprietary chromatographic techniques to ensure high purity and functionality . This recombinant form is used in various research and development applications, including studies on protein-protein interactions, signal transduction pathways, and the ubiquitin-proteasome system.
The availability of recombinant human COPS8 has facilitated numerous studies aimed at understanding the intricate mechanisms of the CSN complex and its role in cellular regulation. Researchers utilize recombinant COPS8 to:
- Investigate the structural and functional aspects of the CSN complex.
- Study the interactions between CSN subunits and other cellular proteins.
- Explore the regulatory mechanisms of protein degradation and their implications in diseases such as cancer.
