COPS7A Human

COP9 Signalosome Subunit 7A Human Recombinant
Cat. No.
BT19073
Source
Escherichia Coli.
Synonyms
COP9 Signalosome Subunit 7A, COPS7A, Dermal Papilla-Derived Protein 10, CSN7A, SGN7a, JAB1-Containing Signalosome Subunit 7a, COP9 Complex Subunit 7a, COP9 Constitutive Photomorphogenic Homolog Subunit 7A, COP9 Signalosome Complex Subunit 7a, DERP10, Signalosome Subunit 7a.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 80.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

COPS7A Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 298 amino acids (1-275) and having a molecular mass of 32.7 kDa.
COPS7A is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
COP9 Signalosome Subunit 7A (COPS7A), a part of the COP9 signalosome complex (CSN), plays a role in various cellular and developmental processes. The CSN complex is crucial for regulating the ubiquitin (Ubl) conjugation pathway. It does this by mediating the deneddylation of cullin subunits within SCF-type E3 ligase complexes. This action reduces the Ubl ligase activity of SCF-type complexes like SCF, CSA, and DDB2. Additionally, this complex participates in phosphorylating proteins like p53/TP53, JUN, I-kappa-B-alpha/NFKBIA, ITPK1, and IRF8/ICSBP, likely through its connection with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN respectively stimulates and safeguards against degradation by the Ubl system.
Description
Recombinant human COPS7A, produced in E.coli, is a single, non-glycosylated polypeptide chain. It consists of 298 amino acids (residues 1-275) and has a molecular weight of 32.7 kDa. A 23 amino acid His-tag is fused to the N-terminus of COPS7A. Purification is achieved using proprietary chromatographic methods.
Physical Appearance
A clear solution that has been sterilized by filtration.
Formulation
The COPS7A solution is provided at a concentration of 0.5mg/ml. It is prepared in a buffer containing 20mM Tris-HCl (pH 8.0), 0.4M Urea, and 10% glycerol.
Stability
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to store the product frozen at -20°C. To ensure optimal stability during long-term storage, consider adding a carrier protein (0.1% HSA or BSA). Repeated freezing and thawing of the product should be avoided.
Purity
The purity of the product, as determined by SDS-PAGE analysis, is greater than 80.0%.
Synonyms
COP9 Signalosome Subunit 7A, COPS7A, Dermal Papilla-Derived Protein 10, CSN7A, SGN7a, JAB1-Containing Signalosome Subunit 7a, COP9 Complex Subunit 7a, COP9 Constitutive Photomorphogenic Homolog Subunit 7A, COP9 Signalosome Complex Subunit 7a, DERP10, Signalosome Subunit 7a.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMSAEVKV TGQNQEQFLL LAKSAKGAAL ATLIHQVLEA PGVYVFGELL DMPNVRELAE SDFASTFRLL TVFAYGTYAD YLAEARNLPP LTEAQKNKLR HLSVVTLAAK VKCIPYAVLL EALALRNVRQ LEDLVIEAVY ADVLRGSLDQ RNQRLEVDYS IGRDIQRQDL SAIARTLQEW CVGCEVVLSG IEEQVSRANQ HKEQQLGLKQ QIESEVANLK KTIKVTTAAA AAATSQDPEQ HLTELREPAP GTNQRQPSKK ASKGKGLRGS AKIWSKSN.

Product Science Overview

Introduction

The COP9 Signalosome Subunit 7A (COPS7A) is a crucial component of the COP9 signalosome (CSN) complex, which is an evolutionarily conserved multi-subunit protease. This complex plays a significant role in regulating the activity of the ubiquitin conjugation pathway, which is essential for various cellular processes, including protein degradation, cell cycle control, and signal transduction .

Structure and Composition

The COP9 signalosome complex is composed of several subunits, including CSN1 (COPS1), CSN2 (COPS2), CSN3 (COPS3), CSN4 (COPS4), CSN5 (COPS5), CSN6 (COPS6), CSN7A (COPS7A), and CSN8 (COPS8). The CSN7A subunit contains a PCI (Proteasome CSN9 initiation factor 3) region and a coiled-coil region, which are crucial for its interaction with other subunits of the complex .

Function

The primary function of the COP9 signalosome is to regulate the ubiquitin conjugation pathway by mediating the deneddylation of SCF-type E3 ligase complexes. This deneddylation process leads to a decrease in the ubiquitin ligase activity of SCF-complexes, thereby controlling the degradation of various proteins . Additionally, the COP9 signalosome is involved in the phosphorylation of several key proteins, including p53, c-Jun, IκB-α, and IRF-8. The phosphorylation of these proteins by the CSN complex protects them from degradation by the ubiquitin-proteasome system .

Expression and Localization

COPS7A is widely expressed in various tissues, including lymphoid tissue, bone marrow, testis, skeletal muscle, and several others. It is detected in both the nucleus and the cytoplasm, functioning as both a monomer and an obligate subunit of the CSN complex .

Clinical Significance

Mutations or dysregulation of the COP9 signalosome subunits, including COPS7A, have been associated with several diseases and developmental disorders. For instance, alterations in the CSN complex can lead to defects in cell cycle control and signal transduction, contributing to the development of cancer and other proliferative diseases .

Research and Applications

Recombinant COPS7A protein is widely used in research to study the function and regulation of the COP9 signalosome complex. It is also utilized in various biochemical assays to investigate the interactions between different subunits of the CSN complex and their role in cellular processes .

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