COPS6 Human
COP9 Constitutive Photomorphogenic 6 Human Recombinant
Cat. No.
BT18975
Source
Escherichia Coli.
Synonyms
CSN6, MOV34-34KD, COP9 signalosome complex subunit 6, JAB1-containing signalosome subunit 6, MOV34 homolog, Vpr-interacting protein, hVIP, COPS6.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 80.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
COPS6 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 347 amino acids (1-327 a.a.) and having a molecular mass of 38.9kDa.
COPS6 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
COPS6 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
COP9 Constitutive Photomorphogenic 6 (COPS6), a member of the translation initiation factor 3 (eIF3) superfamily, peptidase M67A family, and CSN6 subfamily, constitutes one of the eight subunits of the COP9 signalosome. This highly conserved protein complex plays a crucial role in regulating multiple signaling pathways. Functionally and structurally similar to the 19S regulatory particle of the 26S proteasome, the COP9 signalosome interacts with SCF-type E3 ubiquitin ligases, acting as a positive regulator. Additionally, it participates in cell cycle regulation and potentially serves as a cellular cofactor for the HIV-1 accessory gene product Vpr.
Description
Recombinant Human COPS6, expressed in E. coli, is a non-glycosylated polypeptide chain consisting of 347 amino acids (specifically, amino acids 1 to 327). With a molecular weight of 38.9 kDa, this protein is engineered with a 20 amino acid His-tag fused at its N-terminus. Purification is achieved through proprietary chromatographic techniques.
Physical Appearance
The product is a clear solution that has undergone sterile filtration.
Formulation
The COPS6 protein solution (provided at a concentration of 0.25 mg/ml) is formulated in a buffer containing 20 mM Tris-HCl (pH 8.0), 0.4 M urea, and 10% glycerol.
Stability
For optimal storage, the product should be kept at 4°C if it will be used within 2-4 weeks. For longer storage durations, freezing at -20°C is recommended. To further enhance long-term stability, consider adding a carrier protein (either HSA or BSA) at a concentration of 0.1%. It is crucial to avoid subjecting the product to repeated cycles of freezing and thawing.
Purity
Analysis by SDS-PAGE indicates a purity exceeding 80.0%.
Synonyms
CSN6, MOV34-34KD, COP9 signalosome complex subunit 6, JAB1-containing signalosome subunit 6, MOV34 homolog, Vpr-interacting protein, hVIP, COPS6.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MAAAAAAAAA TNGTGGSSGM EVDAAVVPSV MACGVTGSVS VALHPLVILN ISDHWIRMRS QEGRPVQVIG ALIGKQEGRN IEVMNSFELL SHTVEEKIII DKEYYYTKEE QFKQVFKELE FLGWYTTGGP PDPSDIHVHK QVCEIIESPL FLKLNPMTKH TDLPVSVFES VIDIINGEAT MLFAELTYTL ATEEAERIGV DHVARMTATG SGENSTVAEH LIAQHSAIKM LHSRVKLILE YVKASEAGEV PFNHEILREA YALCHCLPVL STDKFKTDFY DQCNDVGLMA YLGTITKTCN TMNQFVNKFN VLYDRQGIGR RMRGLFF.
Product Science Overview
Structure and Composition
Function and Role
COPS6, as part of the CSN complex, is involved in multiple signaling pathways . It acts as a regulator in various cellular processes, including:
- Cell Cycle Regulation: COPS6 is involved in the regulation of the cell cycle, ensuring proper cell division and function .
- Protein Degradation: The CSN complex, including COPS6, plays a role in the ubiquitin-proteasome pathway, which is crucial for protein degradation and turnover .
- Signal Transduction: COPS6 is implicated in various signaling pathways, influencing how cells respond to external stimuli .
Recombinant Human COPS6
Recombinant human COPS6 is a form of the protein that is produced through recombinant DNA technology. This involves inserting the gene that encodes COPS6 into a host organism, such as E. coli, to produce the protein in large quantities . The recombinant protein is often tagged with a His-tag to facilitate purification and detection .
Applications
Recombinant human COPS6 is used in various research applications, including:
- Studying Protein Interactions: Researchers use recombinant COPS6 to study its interactions with other proteins and its role in the CSN complex .
- Drug Development: Understanding the function of COPS6 can aid in the development of drugs targeting diseases related to cell cycle dysregulation and protein degradation .
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