CNDP1 produced in Sf9
Insect cells is a single, glycosylated polypeptide chain containing 489 amino
acids (27-507a.a.) and having a molecular mass of 54.9kDa. (Molecular size on
SDS-PAGE will appear at approximately 50-70kDa). CNDP1 is expressed with an 8 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques. |
CNDP1, produced in Sf9 insect cells, is a single, glycosylated polypeptide chain containing 489 amino acids (27-507a.a.) and has a molecular mass of 54.9 kDa. On SDS-PAGE, the molecular size will appear at approximately 50-70 kDa. This protein is expressed with an 8 amino acid His tag at the C-terminus and is purified by proprietary chromatographic techniques. |
The CNDP1 gene contains a trinucleotide (CTG) repeat length polymorphism in its coding region . This polymorphism has been associated with susceptibility to various conditions, including diabetic nephropathy . The recombinant human CNDP1 protein is typically expressed in systems such as HEK 293 cells or Baculovirus . The protein is often tagged with a His-tag at the C-terminus to facilitate purification and detection .
CNDP Dipeptidase 1 primarily functions to hydrolyze dipeptides, specifically carnosine (β-alanyl-L-histidine), into its constituent amino acids . Carnosine is known for its antioxidant properties and its role in buffering pH in muscles . By breaking down carnosine, CNDP1 helps regulate its levels in tissues and fluids.
Variations in the CNDP1 gene have been linked to different health conditions. For instance, certain polymorphisms are associated with a higher risk of developing diabetic nephropathy, a complication of diabetes that affects the kidneys . Understanding the function and regulation of CNDP1 can provide insights into potential therapeutic targets for such conditions.
Recombinant human CNDP1 is produced using various expression systems to ensure high purity and activity. The protein is often purified to >95% purity and has low endotoxin levels (<1 EU/µg), making it suitable for research applications . It is used in studies to understand the enzyme’s structure, function, and role in disease.