CNDP Dipeptidase 2, also known as Cytosolic non-specific dipeptidase 2 (CNDP2), is a human enzyme that belongs to the M20 family of metallopeptidases. It is also referred to as carnosine dipeptidase II, CN2, or carboxypeptidase of glutamate-like (CPGL). This enzyme plays a crucial role in protein and peptide metabolism by hydrolyzing dipeptides into their constituent amino acids .
CNDP2 is a cytosolic enzyme that functions as a homodimer, with each subunit containing a catalytic domain that binds two metal ions, either Mn²⁺ or Zn²⁺. These metal ions are essential for the enzyme’s activity and substrate specificity . CNDP2 is unique in its ability to catalyze the formation of pseudodipeptides of lactic acid and amino acids through reverse proteolysis in vivo .
The CNDP2 gene is ubiquitously expressed in various tissues, with higher levels observed in the kidney and liver . It is also expressed in histaminergic neurons in the tuberomammillary nucleus, suggesting a role in histamine synthesis . The enzyme is localized in the cytoplasm and has been detected in both the cytoplasm and nucleus of cells .