CNDP1 Human, Active
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Description
CNDP1 is expressed with a 8 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
CNDP Dipeptidase 1, also known as carnosine dipeptidase 1, is an enzyme encoded by the CNDP1 gene in humans. This enzyme belongs to the M20 metalloprotease family and is primarily expressed in the brain. It is a homodimeric dipeptidase, meaning it forms a dimer consisting of two identical subunits. The recombinant form of this enzyme is produced using various expression systems, such as HEK 293 cells or Sf9 insect cells, to ensure high purity and biological activity.
The CNDP Dipeptidase 1 enzyme is composed of 489 amino acids and has a molecular mass of approximately 54.9 kDa . The enzyme contains a His tag at the C-terminus, which facilitates its purification. The three-dimensional structure of CNDP Dipeptidase 1 has been determined using techniques such as X-ray crystallography, providing insights into its active site and catalytic mechanism .
CNDP Dipeptidase 1 catalyzes the hydrolysis of dipeptides, specifically those containing a histidine residue at the C-terminus. It exhibits the highest activity towards carnosine (beta-alanyl-L-histidine) and anserine (beta-alanyl-3-methyl-histidine) . The enzyme plays a crucial role in the metabolism of these dipeptides, breaking them down into their constituent amino acids.
The recombinant form of CNDP Dipeptidase 1 is biologically active and has been shown to have a specific activity of greater than 3,000 pmol/min/µg, as measured by the hydrolysis of carnosine at pH 6.8 and 25°C . This high level of activity makes it suitable for various research applications, including studies on dipeptide metabolism and the role of carnosine in physiological processes.
CNDP Dipeptidase 1 is primarily expressed in the brain and is involved in the metabolism of carnosine and related dipeptides . Carnosine is a dipeptide with antioxidant properties and is thought to play a role in protecting cells from oxidative stress. The enzyme’s activity helps regulate the levels of carnosine in tissues, ensuring proper cellular function and protection against damage.
Mutations in the CNDP1 gene can lead to a metabolic disorder known as carnosinemia, characterized by elevated levels of carnosine in the blood and urine . This condition can result in neurological symptoms and developmental delays. Additionally, variations in the CNDP1 gene have been associated with a reduced risk of diabetic nephropathy, a complication of diabetes that affects the kidneys .
Recombinant CNDP Dipeptidase 1 is widely used in research to study the metabolism of dipeptides and the role of carnosine in various physiological processes. It is also used to investigate the effects of carnosine on cellular function and its potential therapeutic applications in conditions such as diabetes and neurodegenerative diseases .
