The recombinant form of CNDP1 from mice is typically produced with a C-terminal His-tag and corresponds to the amino acids 1-492 of the native mouse protein . The enzyme is expressed using a baculovirus system in insect cells, which allows for proper folding and post-translational modifications . The molecular weight of the recombinant mouse CNDP1 is approximately 56.1 kDa .
CNDP1 functions by degrading carnosine and homocarnosine, which are dipeptides found in human plasma and brain . This degradation process is crucial for maintaining the balance of these dipeptides, which play roles in protecting cells from oxidative stress and regulating neurotransmitter activity .
Recombinant CNDP1 is widely used in research to study its role in various physiological processes and its potential therapeutic applications. For instance, it has been investigated for its neuroprotective properties and its involvement in metabolic pathways related to diabetes and kidney function .
The enzyme’s activity is measured by its ability to cleave carnosine in a two-step assay, with a specific activity greater than 250 pmoles/min/μg . This high activity makes it a valuable tool for biochemical studies and drug development.
Recombinant CNDP1 is typically stored at 4°C for short-term use and at -20°C for long-term storage to prevent degradation . It is supplied in a buffer containing PBS (pH 7.4) and 10% glycerol, without any preservatives . Proper storage and handling are essential to maintain the enzyme’s stability and activity.