CMV Pp52

Cytomegalovirus (CMV) is a member of the Betaherpesvirinae subfamily of Herpesviridae, which includes herpes simplex viruses. CMV is a double-stranded DNA virus that can establish latent infections and cause disease, particularly in immunocompromised individuals . One of the key proteins involved in CMV replication is the pp52 (UL44) protein.

The pp52 (UL44) protein is a nonstructural phosphoprotein that plays a crucial role in CMV replication. It binds to double-stranded DNA and acts as an essential accessory protein for viral gene expression . The UL44 promoter has a complex structure with three TATA elements, one of which is active only at late times after infection, making it necessary for efficient viral growth during CMV infection .

Recombinant CMV pp52 (UL44) is produced using E. coli expression systems. The recombinant protein typically contains the immunodominant regions of CMV pp52, specifically amino acids 202-434, and is often fused to a GST (Glutathione S-transferase) tag for purification purposes . The recombinant protein is purified using proprietary chromatographic techniques to achieve high purity levels .

Recombinant CMV pp52 (UL44) is used in various research applications, including:

• ELISA (Enzyme-Linked Immunosorbent Assay): The recombinant protein is suitable for use in ELISA to detect antibodies against CMV in sera from infected individuals .
• Immunological Studies: The protein is immunoreactive with sera from CMV-infected individuals, making it useful for immunological studies .
• Vaccine Development: Research on recombinant CMV pp52 (UL44) contributes to the development of vaccines and therapeutic interventions against CMV .

Recombinant CMV pp52 (UL44) is typically stored at -20°C to maintain its stability. It is important to avoid freeze/thaw cycles to preserve the protein’s integrity. The product is stable for up to six months under proper storage conditions .