CMV Pp150
Description
Product Specs
Product Science Overview
Cytomegalovirus (CMV) is a member of the Betaherpesvirinae subfamily of Herpesviridae, which also includes herpes simplex virus types 1 and 2, varicella-zoster virus, and Epstein-Barr virus . CMV is a double-stranded linear DNA virus with a large genome ranging from 230-240 kilobase pairs . It has the unique ability to remain latent over long periods and can reactivate under certain conditions .
CMV Pp150 (UL32) is a major tegument protein of the virus, playing a crucial role in the virus’s life cycle. The protein is a 150-kDa phosphoprotein that is essential for the cell cycle and differentiation state-dependent onset of human cytomegalovirus (HCMV) lytic gene expression . The protein binds to cyclin A2 via a functional RXL/Cy motif, resulting in its cyclin A2-dependent phosphorylation . This interaction is critical for the virus to escape the cyclin-dependent kinase (CDK)-mediated block of immediate early (IE) gene expression in the S/G2 phase .
Recombinant CMV Pp150 (UL32) is produced using E. coli as the expression system . The recombinant protein contains the immunodominant regions of CMV Pp150, specifically amino acids 1011-1048 . It is purified using proprietary chromatographic techniques to achieve a purity of over 95%, as determined by 10% PAGE (Coomassie staining) and RP-HPLC .
The recombinant CMV Pp150 is formulated in a solution containing 25 mM Tris-HCl pH 8.0, 1 mM EDTA, and 50% glycerol . It is stable for up to five years when frozen and for one month in solution at room temperature . The protein is immunoreactive with sera from CMV-infected individuals and can be used in ELISA and Western blots for the detection of CMV with minimal specificity problems .
