CMV Pp28

Cytomegalovirus (CMV) is a member of the herpesvirus family, known for its ability to cause lifelong infections in humans. One of the key proteins involved in CMV’s lifecycle is the phosphoprotein 28 (pp28), also known as UL99. This protein plays a crucial role in the assembly and maturation of the virus.

The pp28 protein is a myristylated phosphoprotein that is a constituent of the virion. It is positioned within the tegument of the virus particle, a protein structure that resides between the capsid and envelope . In infected cells, pp28 is found in a cytoplasmic compartment derived from the Golgi apparatus, where the virus buds into vesicles to acquire its final membrane .

The primary function of pp28 is to facilitate the cytoplasmic envelopment of tegument-associated capsids, which is essential for the production of infectious virus particles . The protein contains immunodominant regions, specifically amino acids 130-160, which are crucial for its function .

Recombinant pp28 is typically produced in E. coli and contains the immunodominant regions of the native protein . This recombinant protein is purified using proprietary chromatographic techniques to ensure high purity and functionality . It is commonly used as an antigen in ELISA and Western blots for the detection of CMV with minimal specificity problems .

Recombinant pp28 has several applications in research and diagnostics:

• Antigen in ELISA and Western Blots: It serves as an excellent antigen for the detection of CMV, providing high specificity and sensitivity .
• Vaccine Development: Due to its immunogenic properties, pp28 is a potential candidate for vaccine development against CMV.
• Research: It is used in various studies to understand the molecular mechanisms of CMV assembly and maturation .