CMV Pp38

Cytomegalo Virus Pp38 (UL80a) Recombinant
Cat. No.
BT1611
Source
Escherichia Coli.
Synonyms
Appearance
Purity
CMV Pp38 protein is >95% pure as determined by 10% PAGE (coomassie staining).
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

The E.Coli derived 52.8kDa recombinant protein contains the CMV Pp38 (UL80a) immunodominant regions, 117-373 amino acids and fused to a GST-Tag at C-terminus.

Product Specs

Introduction
CMV, a member of the Betaherpesvirinae subfamily of Herpesviridae, which also includes herpes simplex virus types 1 and 2, varicella-zoster virus, and Epstein-Barr virus, is known for its characteristic ability to establish latency over extended periods. This double-stranded linear DNA virus possesses 162 hexagonal protein capsomeres enveloped by a lipid membrane. With a genome size ranging from 230 to 240 kilobase pairs, CMV has the largest genome among herpesviruses. Human CMV is characterized by unique and inverted repeats, resulting in four genome isomers due to the inversion of L-S genome components (class E). Its replication process can be categorized into immediate early, delayed early, and late gene expression phases, based on the timing of synthesis post-infection. DNA replication occurs through a rolling circle mechanism. In laboratory settings, CMV replicates in human fibroblasts.
Description
The recombinant CMV Pp38 protein, derived from E. coli, has a molecular weight of 52.8 kDa. It encompasses the immunodominant regions of CMV Pp38 (UL80a), specifically amino acids 117-373, and is fused to a GST-Tag at the C-terminus.
Purity
The purity of CMV Pp38 protein exceeds 95%, as determined by 10% PAGE (coomassie staining).
Formulation
The protein is supplied at a concentration of 1 mg/ml in a buffer solution containing 25 mM Tris-HCl (pH 7.2), 1 mM EDTA, and 50% glycerol.
Stability
For optimal stability, CMV Pp38 protein should be stored at or below -18 °C. While it can remain stable at 4 °C for up to one week, it is recommended to avoid repeated freeze-thaw cycles.
Source
Escherichia Coli.
Purification Method
CMV Pp38 was purified by proprietary chromatographic technique.

Product Science Overview

Introduction

Cytomegalovirus (CMV) is a member of the Betaherpesvirinae subfamily of Herpesviridae, which includes other viruses such as herpes simplex virus types 1 and 2, varicella-zoster virus, and Epstein-Barr virus . CMV is a double-stranded linear DNA virus with a complex structure, including 162 hexagonal protein capsomeres surrounded by a lipid membrane . It has the largest genome among the herpes viruses, ranging from 230-240 kilobase pairs .

CMV Pp38 (UL80a) Protein

The CMV Pp38 (UL80a) protein is a phosphoprotein that plays a crucial role in the virus’s life cycle. It is derived from the E. coli bacterium and contains the immunodominant regions of the CMV Pp38 (UL80a) protein, specifically amino acids 117-373 . This recombinant protein is approximately 52.8 kDa in size and is purified using proprietary chromatographic techniques to achieve a purity of over 95% .

Importance and Applications

CMV Pp38 (UL80a) is considered one of the most common viral infections in humans. While the virus rarely causes disease in healthy individuals, it can persist as a latent or chronic infection in various tissues, including the salivary glands, renal tubules, cervix, and leukocytes . CMV infections can be severe in newborns and immunocompromised individuals, such as patients with AIDS, cancer patients, and organ transplant recipients .

The recombinant CMV Pp38 (UL80a) protein is highly immunoreactive with sera from CMV-infected individuals, making it an excellent antigen for use in ELISA and Western blot assays . These assays are essential for detecting CMV infections with minimal specificity problems .

Medical Conditions Associated with CMV

CMV infections can lead to various medical conditions, including interstitial pneumonia, mononucleosis (heterophile negative), congenital defects, abortion, mental retardation, blindness, and deafness . The transmission of CMV through transfused blood is a significant concern for organ transplant recipients, neonates, and heart surgery patients .

Storage and Stability

The recombinant CMV Pp38 (UL80a) protein is shipped at ambient temperature and should be stored at -20°C upon arrival . It remains stable for up to five years when frozen and for one month in solution at room temperature .

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