CKB Human His

Creatine Kinase (CK) is a crucial enzyme involved in cellular energy homeostasis. It catalyzes the reversible transfer of phosphate between adenosine triphosphate (ATP) and various phosphogens such as creatine phosphate. This enzyme exists in different isoforms, including the brain-specific isoform known as Creatine Kinase Brain (CKB) or CK-BB.

CKB is a cytoplasmic enzyme that primarily functions in the brain but is also found in other tissues. It acts as a homodimer consisting of two identical brain-type CK-B subunits. The enzyme plays a vital role in maintaining energy balance within cells by facilitating the rapid regeneration of ATP from phosphocreatine (PCr) and adenosine diphosphate (ADP) during periods of high energy demand .

Recombinant human CKB with a His tag is a laboratory-produced version of the enzyme. The His tag, a sequence of histidine residues, is added to facilitate purification and detection of the protein. This recombinant protein is typically expressed in E. coli and purified to high levels of purity (>95%) using techniques such as SDS-PAGE .

Recombinant human CKB is used in various research applications, including:

• Functional Assays: To study enzyme kinetics and interactions with other proteins.
• Biochemical Studies: To investigate the role of CKB in cellular energy metabolism.
• Clinical Research: As a marker in blood tests for conditions such as myocardial infarction, rhabdomyolysis, and muscular dystrophy .

The recombinant protein is typically supplied as a lyophilized powder or in a buffered solution. It should be stored at -20°C or below for long-term storage and at 4°C for short-term storage. Repeated freeze-thaw cycles should be avoided to maintain protein stability .