CKMT1 Human
Creatine Kinase Muscle Type-1 Human Recombinant
Cat. No.
BT240
Source
Pichia Pastoris.
Synonyms
Creatine kinase M-type, EC 2.7.3.2, Creatine kinase M chain, M-CK, CKM, CKMM, CKMMITI.
Appearance
Sterile Filtered colourless liquid formulation.
Purity
Greater than 95.0% as determined by
a) Analysis by RP-HPLC
b) Analysis by SDS-PAGE
a) Analysis by RP-HPLC
b) Analysis by SDS-PAGE
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
CKMT1 Human Recombinant without C-terminal Lysine on both chains produced in Pichia Pastoris is a glycosylated 47kDa polypeptide chain having an identical amino acid sequence compared to the native enzyme, purified under non-denaturing conditions and reacts with polyclonal antibodies to MM Isoenzyme in ELISA. The CKMT1 is purified by proprietary chromatographic techniques.
Product Specs
Introduction
Creatine Kinase MM, a cytoplasmic enzyme key to energy regulation, serves as a crucial serum indicator for myocardial infarction. This enzyme facilitates the reversible phosphate transfer between ATP and various phosphogens like creatine phosphate. Functioning as a homodimer in striated muscle and other tissues, it also forms a heterodimer with a similar brain isozyme in the heart. Notably, this enzyme belongs to the ATP:guanido phosphotransferase protein family.
Description
Produced in Pichia Pastoris, recombinant human CKMT1, devoid of the C-terminal Lysine on both chains, is a glycosylated polypeptide weighing 47kDa. This protein shares an identical amino acid sequence with the native enzyme. Purified in non-denaturing conditions, it exhibits reactivity with polyclonal antibodies specific to MM Isoenzyme in ELISA. The purification of CKMT1 is achieved using proprietary chromatographic methods.
Physical Appearance
Clear, colorless liquid solution, sterile-filtered.
Formulation
The formulation of CKMT1 consists of 0.01M Tris-HCl, 0.075M NaCl, 10mM beta-mercaptoethanol, 50% glycerol, 0.1% sodium azide, at a pH of 7.2.
Stability
While CKMT1 remains stable at 15°C for up to 7 days, it is recommended to store it below -18°C. It's essential to avoid repeated freeze-thaw cycles.
Purity
Purity exceeds 95.0% as determined by:
a) Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC) analysis
b) Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) analysis
Biological Activity
Measured using the enzymatic activity of Creatine phosphokinase (procedure No.45-UV), the biological activity, expressed as 1IU being equivalent to 1 micromole of creatine phosphate, was found to be 537IU/mg at 37 degrees Celsius. This corresponds to a specific activity of 1,863ng/ml.
Synonyms
Creatine kinase M-type, EC 2.7.3.2, Creatine kinase M chain, M-CK, CKM, CKMM, CKMMITI.
Source
Pichia Pastoris.
Product Science Overview
Types of Creatine Kinase
There are three main isoforms of CK:
- CK-MM (Muscle type): Predominantly found in skeletal muscle and heart tissue.
- CK-MB (Brain type): Found in the brain and other tissues.
- CK-BB (Hybrid type): Found in various tissues, including the heart.
Creatine Kinase Muscle Type-1 (CK-MM)
Human Recombinant CK-MM
Recombinant human CK-MM is produced using genetic engineering techniques. The gene encoding CK-MM is inserted into a suitable expression system, such as E. coli, to produce the enzyme in large quantities. This recombinant enzyme is used in various research and clinical applications, including:
- Biochemical studies: Understanding the enzyme’s structure, function, and kinetics.
- Clinical diagnostics: Measuring CK-MM levels in blood samples to diagnose and monitor muscle-related diseases and conditions, such as muscular dystrophy and myocardial infarction .
- Therapeutic research: Investigating potential treatments for muscle-related disorders.
Production and Purification
The production of recombinant CK-MM involves several steps:
- Gene cloning: The CK-MM gene is cloned into an expression vector.
- Transformation: The vector is introduced into a host organism, such as E. coli.
- Expression: The host organism is cultured under conditions that induce the expression of the CK-MM protein.
- Purification: The recombinant CK-MM protein is purified using techniques such as affinity chromatography to achieve high purity and activity levels .
Applications of Recombinant CK-MM
Recombinant CK-MM has several applications in research and medicine:
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