CKMT3 Human
Creatine Kinase Muscle Type-3 Human Recombinant
Cat. No.
BT451
Source
Pichia Pastoris.
Synonyms
Creatine kinase M-type, EC 2.7.3.2, Creatine kinase M chain, M-CK, CKM, CKMM, CKMMITIII.
Appearance
Sterile Filtered colorless liquid formulation.
Purity
Greater than 95.0% as determined by
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
CKMT3 Human Recombinant produced in Pichia Pastoris is a glycosylated polypeptide chain having an identical amino acid sequence compared to the native enzyme, purified under non-denaturing conditions and reacts with polyclonal antibodies to MM Isoenzyme in ELISA.The CKMT3 is purified by proprietary chromatographic techniques.
Product Specs
Introduction
Creatine Kinase (CK) exists as three isoenzymes (MM, MB, and BB) distributed in muscle, cardiac, and brain tissues. These recombinant CK proteins serve as valuable tools for calibrating diagnostic instruments and supporting research on neuromuscular diseases. Their applications span a range of neuromuscular disorders, including cardiac diseases, mitochondrial disorders, inflammatory myopathies, myasthenia gravis, polymyositis, McArdle's disease, neuromuscular junction disorders, muscular dystrophy, amyotrophic lateral sclerosis (ALS), thyroid disorders, central core disease, acid maltase deficiency, myoglobinuria, rhabdomyolysis, motor neuron diseases, rheumatic diseases, and other conditions characterized by abnormal creatine kinase levels.
Description
CKMT3 Human Recombinant is a glycosylated polypeptide chain produced in Pichia Pastoris. This recombinant protein exhibits an identical amino acid sequence to the native CKMT3 enzyme. Purified under non-denaturing conditions, it demonstrates reactivity with polyclonal antibodies specific to the MM isoenzyme in ELISA assays. The purification process for CKMT3 involves proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered colorless liquid.
Formulation
The protein formulation contains 20mM Tris pH 8.0, 1mM EDTA, and 1mM DTT for each mg of protein.
Stability
While CKMT3 remains stable at 15°C for up to 7 days, storage below -18°C is recommended. Repeated freeze-thaw cycles should be avoided.
Purity
The purity is greater than 95.0% as determined by: (a) Reverse-phase high-performance liquid chromatography (RP-HPLC) analysis and (b) Sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis.
Biological Activity
The biological activity, assessed using the Creatine Phosphokinase assay procedure No. 45-UV (1 IU = 1 µmole creatine phosphate), was determined to be 500 IU/mg at 37°C. This corresponds to a specific activity of 2,000 ng/ml.
Synonyms
Creatine kinase M-type, EC 2.7.3.2, Creatine kinase M chain, M-CK, CKM, CKMM, CKMMITIII.
Source
Pichia Pastoris.
Product Science Overview
Isoforms of Creatine Kinase
Creatine Kinase Muscle Type-3 (CKMT3)
Human Recombinant CKMT3
Recombinant human CKMT3 is produced using E. coli expression systems. This recombinant protein is used for various research and diagnostic purposes, including:
Applications and Importance
The recombinant form of CKMT3 is highly purified and has a specific activity that makes it suitable for biochemical assays. It is used in:
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