CKB Human

Creatine Kinase Brain Human Recombinant
Cat. No.
BT9408
Source
E.coli.
Synonyms
Creatine kinase B-type, B-CK, BCK, CKBB, HEL-211, HEL-S-29, CKB, EC 2.7.3.2, Creatine kinase B chain, CKB, CKBB, CKBBI.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

CKB Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 381 amino acids (1-381a.a) and having a molecular mass of 42.6kDa.
CKB is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Creatine Kinase BB is a cytoplasmic enzyme that plays a role in maintaining energy balance within cells. This enzyme facilitates the reversible transfer of phosphate between ATP, the primary energy currency of cells, and phosphogens like creatine phosphate. It functions as a homodimer in the brain and other tissues, and as a heterodimer with a similar muscle isozyme in the heart. Creatine Kinase BB belongs to the ATP:guanido phosphotransferase protein family. A pseudogene of this gene has also been identified.
Description
Recombinant human CKB, produced in E. coli, is a single polypeptide chain that lacks glycosylation. It consists of 381 amino acids (1-381a.a) and has a molecular weight of 42.6kDa. The purification of CKB is achieved using specialized chromatographic methods.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The CKB solution is provided at a concentration of 1mg/ml and contains the following components: 20mM Tris-Hcl Buffer (pH 8.0), 10% glycerol, and 1mM DTT.
Stability
For short-term storage (2-4 weeks), the CKB solution should be kept refrigerated at 4°C. For extended storage, it is recommended to freeze the solution at -20°C. Adding a carrier protein like HSA or BSA (0.1%) is advisable for long-term storage. It is important to avoid repeated freezing and thawing of the solution.
Purity
The purity of CKB is greater than 90% as determined by SDS-PAGE analysis.
Synonyms
Creatine kinase B-type, B-CK, BCK, CKBB, HEL-211, HEL-S-29, CKB, EC 2.7.3.2, Creatine kinase B chain, CKB, CKBB, CKBBI.
Source
E.coli.
Amino Acid Sequence
MPFSNSHNAL KLRFPAEDEF PDLSAHNNHM AKVLTPELYA ELRAKSTPSG FTLDDVIQTG VDNPGHPYIM TVGCVAGDEE SYEVFKDLFD PIIEDRHGGY KPSDEHKTDL NPDNLQGGDD LDPNYVLSSR VRTGRSIRGF CLPPHCSRGE RRAIEKLAVE ALSSLDGDLA GRYYALKSMT EAEQQQLIDD HFLFDKPVSP LLLASGMARD WPDARGIWHN DNKTFLVWVN EEDHLRVISM QKGGNMKEVF TRFCTGLTQI ETLFKSKDYE FMWNPHLGYI LTCPSNLGTG LRAGVHIKLP NLGKHEKFSE VLKRLRLQKR GTGGVDTAAV GGVFDVSNAD RLGFSEVELV QMVVDGVKLL IEMEQRLEQG QAIDDLMPAQ K.

Product Science Overview

Introduction

Creatine kinase (CK) is an enzyme that plays a crucial role in cellular energy homeostasis. It catalyzes the reversible conversion of creatine and adenosine triphosphate (ATP) to phosphocreatine and adenosine diphosphate (ADP). This reaction is vital for the rapid regeneration of ATP, which is essential for various cellular processes, including muscle contraction and intracellular energy transport.

Isoforms of Creatine Kinase

There are three main isoforms of creatine kinase, each predominantly found in different tissues:

  1. CK-MM: Found mainly in skeletal muscle.
  2. CK-MB: Found primarily in cardiac muscle.
  3. CK-BB: Found mainly in the brain and smooth muscle.
Creatine Kinase Brain (CK-BB)

CK-BB is the isoform of creatine kinase that is predominantly expressed in the brain. It plays a significant role in maintaining the energy balance within brain cells, particularly neurons. The high energy demands of the brain make CK-BB essential for proper brain function.

Recombinant Human Creatine Kinase Brain (CK-BB)

Recombinant human creatine kinase brain (CK-BB) is a form of the enzyme that is produced using recombinant DNA technology. This involves inserting the gene encoding CK-BB into a host organism, such as Escherichia coli (E. coli), which then expresses the protein. The recombinant protein is subsequently purified for use in research and clinical applications.

Production and Purification

The production of recombinant CK-BB typically involves the following steps:

  1. Gene Cloning: The gene encoding human CK-BB is cloned into an expression vector.
  2. Transformation: The expression vector is introduced into a host organism, such as E. coli.
  3. Expression: The host organism is cultured under conditions that induce the expression of CK-BB.
  4. Purification: The expressed CK-BB protein is purified using techniques such as affinity chromatography.
Applications

Recombinant CK-BB has several important applications:

  1. Research: It is used in studies investigating the role of CK-BB in brain metabolism and neurodegenerative diseases.
  2. Clinical Diagnostics: CK-BB levels can be measured in clinical settings to assess brain damage or disease.
  3. Drug Development: It is used in the development of drugs targeting energy metabolism in the brain.

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