CKB Antibody
Creatine Kinase Brain, Mouse Anti Human
Cat. No.
BT15062
Source
Synonyms
Creatine kinase B-type, EC 2.7.3.2, Creatine kinase B chain, B-CK, CKB, CKBB, CKBBI.
Appearance
Sterile filtered colorless solution.
Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
Product Specs
Introduction
Creatine Kinase BB, also known as CKB, is an enzyme that plays a vital role in cellular energy regulation, particularly in tissues with high energy demands like the brain. It facilitates the reversible transfer of phosphate between ATP, the primary energy currency of cells, and creatine phosphate, a molecule that acts as an energy reservoir. CKB primarily functions as a homodimer in the brain and other tissues, but it can also form heterodimers with similar muscle isozymes in the heart. This enzyme is classified under the ATP:guanido phosphotransferase protein family. Interestingly, a non-functional copy of the CKB gene, termed a pseudogene, has also been identified.
Physical Appearance
The product is a clear and colorless solution that has been sterilized by filtration.
Formulation
This product is provided at a concentration of 1mg/ml and is prepared in a solution containing phosphate buffered saline (PBS) at a pH of 7.4, 10% glycerol, and 0.02% sodium azide as a preservative.
Storage Procedures
For short-term storage of up to one month, the product should be kept at 4°C. For extended storage periods, it is recommended to store the product at -20°C. To maintain product integrity and activity, it is crucial to avoid repeated freeze-thaw cycles.
Stability / Shelf Life
The product has a shelf life of 12 months when stored properly at -20°C. At 4°C, the product is stable for 1 month.
Applications
The CKB antibody has undergone rigorous testing in various applications, including ELISA, Western blot analysis, Flow cytometry, and Immunocytochemistry/Immunofluorescence (ICC/IF), to ensure its specificity and reactivity. However, it is essential to note that optimal working dilutions may vary depending on the specific application and experimental conditions. Therefore, users are advised to perform their own titrations to determine the most effective antibody concentration for their intended use.
Synonyms
Creatine kinase B-type, EC 2.7.3.2, Creatine kinase B chain, B-CK, CKB, CKBB, CKBBI.
Purification Method
CKB antibody was purified from mouse ascitic fluids by protein-A affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Clone
PAT8E4AT.
Immunogen
Anti-human CKB mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human CKB amino acids 1-381 purified from E. coli.
Ig Subclass
Mouse IgG2a heavy chain and k light chain.
Product Science Overview
Creatine Kinase Brain (CK-BB)
CK-BB, also known as brain creatine kinase, is a cytoplasmic enzyme involved in energy metabolism. It is encoded by the CKB gene and is a member of the ATP:guanido phosphotransferase protein family. CK-BB acts as a homodimer in the brain and other tissues, and as a heterodimer with a similar muscle isozyme in the heart . The enzyme is crucial for maintaining energy homeostasis in cells with high and fluctuating energy demands, such as neurons .
Mouse Anti-Human CK-BB Antibody
Mouse anti-human CK-BB antibodies are monoclonal antibodies produced by immunizing mice with human CK-BB protein. These antibodies are highly specific and are used in various research applications, including Western blotting, immunohistochemistry, and ELISA. They are valuable tools for studying the expression and function of CK-BB in different tissues and under various physiological and pathological conditions .
Applications and Importance
- Research: Mouse anti-human CK-BB antibodies are extensively used in research to investigate the role of CK-BB in energy metabolism, neurodegenerative diseases, and other conditions affecting the brain and other tissues .
- Diagnostics: These antibodies can be used in diagnostic assays to detect CK-BB levels in biological samples, which can provide insights into various diseases and conditions .
- Therapeutics: Understanding the function and regulation of CK-BB can lead to the development of therapeutic strategies for diseases involving energy metabolism dysregulation .
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