CHGA Human

Chromogranin-A is a large protein with a molecular weight of approximately 86 kDa . It contains multiple dibasic cleavage sites, which allow it to be processed into several smaller, biologically active peptides, including vasostatin, pancreastatin, and parastatin . These peptides act as autocrine or paracrine modulators, influencing the neuroendocrine system by inhibiting hormone and neurotransmitter release .

Recombinant human Chromogranin-A is produced using human embryonic kidney cells (HEK293) as the expression system . The recombinant protein typically includes a C-terminal 6-His tag to facilitate purification and detection . The protein is purified to a high degree, with a purity greater than 90% as determined by SDS-PAGE and visualized by Coomassie Blue staining .

Recombinant Chromogranin-A is used in various research applications, including studies on neuroendocrine tumors, cardiovascular diseases, and metabolic disorders . It is also employed in functional assays, such as ELISA, to study its binding properties and interactions with other proteins .

The recombinant protein is typically lyophilized from a filtered solution in PBS and should be reconstituted at a concentration of 200 μg/mL in PBS . For optimal stability, it should be stored at -20 to -70°C and should avoid repeated freeze-thaw cycles .