CHGA Antibody

Chromogranin-A, Mouse Anti Human
Cat. No.
BT14640
Source
Synonyms
CGA, CHGA, Vasostatin-2, Pituitary secretory protein I, SP-I.
Appearance
Sterile Filtered colorless solution.
Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Product Specs

Introduction
Chromogranin-A (CHGA) belongs to the granin family of neuroendocrine secretory proteins. It is found within secretory vesicles of neurons and endocrine cells. CHGA serves as a precursor to several biologically active peptides, including vasostatin, pancreastatin, and parastatin. These peptides function as autocrine or paracrine negative regulators of the neuroendocrine system. Additional peptides derived from the full-length protein include chromostatin, beta-granin, WE-14, and GE-25. CHGA exhibits diverse biological activities across various tissues and organs, exerting a broad range of homeostatic effects. These include antifungal and antimicrobial properties, modulation of cell adhesion, and inhibition of parathyroid hormone secretion.
Physical Appearance
Clear, colorless solution, sterile-filtered.
Formulation
Supplied as a 1 mg/mL solution in phosphate-buffered saline (PBS) at pH 7.4, containing 0.1% sodium azide as a preservative.
Storage Procedures
For short-term storage (up to 1 month), keep at 4°C. For extended storage, store at -20°C. Avoid repeated freeze-thaw cycles.
Stability / Shelf Life
The product is stable for 12 months when stored at -20°C and for 1 month at 4°C.
Applications
This CHGA antibody has undergone testing by ELISA, Western blot, and immunohistochemistry to confirm its specificity and reactivity. However, optimal working dilutions should be determined empirically for each application. The recommended dilution range for Western blotting is 1:500 to 1:1,000, with a starting dilution of 1:500. For immunohistochemistry, the suggested dilution range is 1:100 to 1:200, with a starting dilution of 1:100.
Synonyms
CGA, CHGA, Vasostatin-2, Pituitary secretory protein I, SP-I.
Purification Method
CHGA antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Clone
P3A5AT.
Immunogen
Anti-human CHGA mAb is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human CHGA amino acids 19-131 purified from E. coli.
Ig Subclass
Mouse IgG1 heavy chain and κ light chain.

Product Science Overview

Chromogranin A in Human Biology

CgA is a 49 kDa protein consisting of 457 amino acids and is encoded by the CHGA gene located on chromosome 14 . It is predominantly produced by neuroendocrine cells and is present in various tissues, including the adrenal medulla, pancreatic islets, and the gastrointestinal tract . CgA is released into the bloodstream in response to extracellular stimulation, making it a valuable biomarker for neuroendocrine tumors .

Diagnostic and Research Applications

CgA is widely used as a biomarker in the diagnosis and prognosis of neuroendocrine tumors, such as carcinoid tumors, pheochromocytomas, and paragangliomas . Its high specificity for neuroendocrine cells makes it an excellent marker for identifying these tumors through immunohistochemistry (IHC) and other diagnostic techniques .

Mouse Anti-Human Chromogranin A Antibody

The Mouse Anti-Human Chromogranin A antibody is a monoclonal antibody developed to specifically detect human CgA. This antibody is produced by immunizing mice with recombinant human CgA protein, leading to the generation of hybridoma cells that produce the desired monoclonal antibody . The antibody is then purified from the ascites fluid of these mice and characterized for its specificity and sensitivity .

Applications in Research and Diagnostics

The Mouse Anti-Human Chromogranin A antibody is used in various research and diagnostic applications, including:

  • Immunohistochemistry (IHC): This technique involves staining tissue sections with the antibody to detect the presence and distribution of CgA in neuroendocrine cells .
  • Western Blotting: The antibody is used to detect CgA in protein samples separated by gel electrophoresis .
  • Immunocytochemistry/Immunofluorescence (ICC/IF): These techniques involve staining cells with the antibody to visualize CgA localization within cells .

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