CHGA Human, HEK
HEK293 cells.
CHGA, CGA, Chromogranin-A, Vasostatin I, SP-I, Pituitary secretory protein I.
Greater than 95.0% as determined by SDS-PAGE.
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Description
CHGA Human Recombinant is a single, glycosylated polypeptide chain containing 445 amino acids (19-457a.a) and having a molecular mass of 49.7kDa (calculated). CHGA is fused to a 6 a.a His tag at C-terminal.
Product Specs
Chromogranin A (CgA) is a protein found in neuroendocrine cells throughout the body. These cells are responsible for releasing hormones and other signaling molecules. CgA is particularly abundant in the digestive system, adrenal glands, and pancreas. Measuring CgA levels is valuable in diagnosing and monitoring neuroendocrine tumors (NETs), which are cancers that arise from these cells. Elevated CgA levels can indicate the presence and aggressiveness of NETs, helping healthcare professionals make informed treatment decisions.
This product consists of a specifically designed version of the human Chromogranin A (CgA) protein produced in a laboratory setting using HEK cells. This recombinant CgA protein encompasses amino acids 19 to 457 of the full-length human CgA sequence, resulting in a single polypeptide chain with a molecular weight of 49.7 kDa. A 6-amino acid Histidine tag is attached to the C-terminus of the protein to facilitate its purification and detection. Notably, this recombinant CgA protein undergoes glycosylation, a process of adding sugar molecules, making it structurally similar to the naturally occurring CgA found in the human body.
The CHGA protein has undergone filtration using a 0.4 µm filter and subsequent lyophilization from a solution containing 0.5mg/ml CHGA in phosphate-buffered saline (PBS) at a pH of 7.0.
To create a working solution of around 0.5 mg/ml, it's advised to introduce deionized water to the lyophilized pellet and allow it to fully dissolve.
For long-term storage, maintain the lyophilized protein at -20°C. After reconstituting the protein, divide it into smaller portions to minimize freeze-thaw repetitions. The reconstituted protein can be kept at 4°C for a limited period, showing stability for up to two weeks without noticeable changes.
The purity of this product is greater than 95%, as assessed by SDS-PAGE, a widely used technique for determining protein purity.
CHGA, CGA, Chromogranin-A, Vasostatin I, SP-I, Pituitary secretory protein I.
HEK293 cells.
LPVNSPMNKG DTEVMKCIVE VISDTLSKPS PMPVSQECFE TLRGDERILS ILRHQNLLKE LQDLALQGAK ERAHQQKKHS GFEDELSEVL ENQSSQAELK EAVEEPSSKD VMEKREDSKE AEKSGEATDG ARPQALPEPM QESKAEGNNQ APGEEEEEEE EATNTHPPAS LPSQKYPGPQ AEGDSEGLSQ GLVDREKGLS AEPGWQAKRE EEEEEEEEAE AGEEAVPEEE GPTVVLNPHP SLGYKEIRKG ESRSEALAVD GAGKPGAEEA QDPEGKGEQE HSQQKEEEEE MAVVPQGLFR GGKSGELEQE EERLSKEWED SKRWSKMDQL AKELTAEKRL EGQEEEEDNR DSSMKLSFRA RAYGFRGPGP QLRRGWRPSS REDSLEAGLP LQVRGYPEEK KEEEGSANRR PEDQELESLS AIEAELEKVA HQLQALRRGH HHHHH
Product Science Overview
Chromogranin A (CgA) is a member of the granin family of proteins, which are found in the secretory vesicles of neuroendocrine cells. It was first identified in the bovine adrenal medulla in 1967 and has since been recognized as a precursor to several functional peptides, including vasostatin-1, vasostatin-2, pancreastatin, catestatin, and parastatin . These peptides play crucial roles in modulating neuroendocrine functions.
Chromogranin A is an intrinsically disordered protein, meaning it does not have a fixed three-dimensional structure under physiological conditions. This characteristic allows it to be cleaved into various bioactive peptides that perform diverse functions. For instance, vasostatin-1 and vasostatin-2 inhibit vasoconstriction, while catestatin acts as an inhibitor of catecholamine release .
Human recombinant Chromogranin A is often expressed in Human Embryonic Kidney (HEK) 293 cells. These cells are widely used in biotechnology for the production of recombinant proteins due to their high transfection efficiency and ability to perform post-translational modifications similar to those in human cells . The recombinant form of Chromogranin A produced in HEK cells is used in various research and clinical applications, including the study of neuroendocrine tumors and cardiovascular diseases .
Chromogranin A serves as a biomarker for several diseases, particularly neuroendocrine tumors (NETs). Elevated levels of circulating CgA can be detected in patients with gastroenteropancreatic NETs and correlate with tumor burden . Additionally, CgA has been implicated in cardiovascular diseases, including essential hypertension, where it may play a role in regulating blood pressure .
The discovery of Chromogranin A marked a significant milestone in the field of neuroendocrinology. Initially identified as a mysterious protein released from the adrenal medulla, it was later found to be a key player in the regulation of neuroendocrine functions. Over the years, research has expanded our understanding of its structure, function, and clinical significance .
