CHGA Human, Sf9
CHGA, CGA, Chromogranin-A, Vasostatin I, SP-I, Pituitary secretory protein I.
Greater than 85.0% as determined by SDS-PAGE.
Description
CHGA produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain (19-457 a.a.) and fused to a 6 aa His Tag at C-terminus containing a total of 448 amino acids and having a molecular mass of 50kDa.CHGA shows multiple bands between 50-70kDa on SDS-PAGE, reducing conditions and purified by proprietary chromatographic techniques.
Product Specs
CHGA, CGA, Chromogranin-A, Vasostatin I, SP-I, Pituitary secretory protein I.
ADLLPVNSPM NKGDTEVMKC IVEVISDTLS KPSPMPVSQE CFETLRGDER ILSILRHQNL LKELQDLALQ GAKERAHQQK KHSGFEDELS EVLENQSSQA ELKEAVEEPS SKDVMEKRED SKEAEKSGEA TDGARPQALP EPMQESKAEG NNQAPGEEEE EEEEATNTHP PASLPSQKYP GPQAEGDSEG LSQGLVDREK GLSAEPGWQA KREEEEEEEE EAEAGEEAVP EEEGPTVVLN PHPSLGYKEI RKGESRSEAL AVDGAGKPGA EEAQDPEGKG EQEHSQQKEE EEEMAVVPQG LFRGGKSGEL EQEEERLSKE WEDSKRWSKM DQLAKELTAE KRLEGQEEEE DNRDSSMKLS FRARAYGFRG PGPQLRRGWR PSSREDSLEA GLPLQVRGYP EEKKEEEGSA NRRPEDQELE SLSAIEAELE KVAHQLQALR RGHHHHHH.
Product Science Overview
CgA is a precursor to numerous bioactive peptides and plays a crucial role in the regulation of neuroendocrine functions. It acts as a negative regulator of the neuroendocrine activity of autocrine or nearby cells (paracrine) . The protein is involved in the formation of secretory granules that contain insulin in pancreatic islet beta cells .
The recombinant form of CgA is produced in Sf9 Baculovirus cells. This recombinant protein is a single, glycosylated polypeptide chain consisting of 448 amino acids, including a 6 amino acid His Tag at the C-terminus . The molecular mass of this recombinant protein is approximately 50 kDa, and it shows multiple bands between 50-70 kDa on SDS-PAGE under reducing conditions .
The recombinant CgA protein solution is formulated in Phosphate Buffered Saline (pH 7.4) with 20% glycerol and 0.1mM PMSF . For short-term storage, it should be kept at 4°C if used within 2-4 weeks. For long-term storage, it is recommended to freeze the protein at -20°C with a carrier protein (0.1% HSA or BSA) to avoid multiple freeze-thaw cycles .
