CHI3L2 Human
Chitinase 3-Like 2 Recombinant
Cat. No.
BT30901
Source
Escherichia Coli.
Synonyms
Chitinase 3-Like 2, Chondrocyte Protein 39, YKL-39, YKL39, Chitinase-3-Like Protein 2, CHIL2, CHI3L2.
Appearance
Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
CHI3L2 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain (Tyr27-Leu390) containing 374 amino acids including a 10 aa His tag at N-terminus. The total calculated molecular mass is 42.1kDa.
Product Specs
Introduction
Chitinase 3-Like 2 (CHI3L2) shares similarities with bacterial chitinases but lacks the enzymatic activity to break down chitin. This secreted protein plays a role in the formation of cartilage. Functioning as a lectin, CHI3L2 exhibits high affinity for binding to chitooligosaccharides and other glycans.
Description
Recombinant Human CHI3L2, produced in E. coli, is a single, non-glycosylated polypeptide chain. It encompasses amino acids Tyr27 to Leu390 (374 amino acids total), including a 10 amino acid Histidine tag located at the N-terminus. The calculated molecular mass is 42.1 kDa.
Physical Appearance
White powder, lyophilized (freeze-dried), and filtered.
Formulation
CHI3L2 was subjected to filtration (0.4 µm) and subsequently lyophilized from a 0.5 mg/ml solution prepared in 30 mM acetate buffer with a pH of 4.
Solubility
To prepare a working stock solution of approximately 0.5 mg/ml, it is recommended to reconstitute the lyophilized pellet in 0.1 M acetate buffer (pH 4.0) and ensure complete dissolution. For adjusting the pH to a higher value, dilute the solution extensively with the appropriate buffer to a concentration of 10 µg/ml. The solubility of this antigen is limited at higher concentrations. It is crucial to note that CHI3L2 is not sterile. Prior to use in cell culture, the product must be sterilized by filtration using an appropriate sterile filter.
Stability
Store the lyophilized protein at -20°C. After reconstitution, aliquot the product to prevent repeated freeze-thaw cycles. The reconstituted protein demonstrates stability at 4°C for a limited period and exhibits no noticeable changes for up to two weeks at this temperature.
Purity
Purity exceeds 95.0% as determined by SDS-PAGE analysis.
Synonyms
Chitinase 3-Like 2, Chondrocyte Protein 39, YKL-39, YKL39, Chitinase-3-Like Protein 2, CHIL2, CHI3L2.
Source
Escherichia Coli.
Amino Acid Sequence
MKHHHHHHASYKLVCYFTNW SQDRQEPGKF TPENIDPFLC SHLIYSFASI ENNKVIIKDK SEVMLYQTIN SLKTKNPKLK ILLSIGGYLF GSKGFHPMVD SSTSRLEFIN SIILFLRNHN FDGLDVSWIY PDQKENTHFT VLIHELAEAF QKDFTKSTKE RLLLTAGVSA GRQMIDNSYQ VEKLAKDLDF INLLSFDFHG SWEKPLITGH NSPLSKGWQD RGPSSYYNVE YAVGYWIHKG MPSEKVVMGI PTYGHSFTLA SAETTVGAPA SGPGAAGPIT ESSGFLAYYE ICQFLKGAKI TRLQDQQVPY AVKGNQWVGY DDVKSMETKV QFLKNLNLGG AMIWSIDMDD FTGKSCNQGP YPLVQAVKRS LGSL.
Product Science Overview
Origin and Secretion
Physiological Activities
Recombinant Production
Applications
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