CHI3L2 Antibody
Chitinase 3-Like 2, Chondrocyte Protein 39, YKL-39, YKL39, Chitinase-3-Like Protein 2, CHIL2, CHI3L2.
Description
Product Specs
Chitinase 3-Like 2 (CHI3L2) shares similarities with bacterial chitinases but does not possess chitinase activity. This secreted protein plays a role in the formation of cartilage. CHI3L2 functions as a lectin, exhibiting high affinity for chitooligosaccharides and various glycans, but not for heparin.
The solution contains 1mg/ml of CHI3L2 antibody in a buffer consisting of PBS at pH 7.4, 10% glycerol, and 0.02% sodium azide.
The CHI3L2 antibody has undergone testing using ELISA and Western blot analysis to confirm its specificity and reactivity. However, due to variations in applications, it is recommended to titrate the reagent for each specific experiment to achieve optimal results.
Chitinase 3-Like 2, Chondrocyte Protein 39, YKL-39, YKL39, Chitinase-3-Like Protein 2, CHIL2, CHI3L2.
CHI3L2 antibody was purified from mouse ascitic fluids by protein-A affinity chromatography.
PAT2B5AT.
Anti-human CHI3L2 mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human CHI3L2 amino acids 27-390 purified from E. coli.
Mouse IgG2b heavy chain and κ light chain.
Product Science Overview
Chitinase 3-Like 2 (CHI3L2) is a member of the chitinase-like proteins (CLPs), which belong to the glycoside hydrolase 18 family . These proteins are structurally similar to chitinases but lack enzymatic activity to hydrolyze chitin. CHI3L2 is also known as YKL-39, derived from the three N-terminal amino acids present on the secreted form and its molecular mass .
The biological function of CHI3L2 is not entirely understood. However, it is known to be involved in various biological processes, including inflammation, extracellular tissue remodeling, and fibrosis . CHI3L2 is expressed in several cell types, including macrophages, chondrocytes, fibroblast-like synovial cells, vascular smooth muscle cells, and hepatic stellate cells .
CHI3L2 has been identified as a novel biomarker in glioblastoma, a highly aggressive brain tumor . Studies have shown that CHI3L2 is highly expressed in the tumor core and peritumoral brain zone (PBZ) of glioblastoma, and its expression is associated with tumor recurrence and poor patient prognosis . The high expression of CHI3L2 in these regions suggests its role in altering the tumor microenvironment and contributing to tumor spread and recurrence .
CHI3L2 is also correlated with immune infiltration in various tumors, including breast cancer and glioblastoma . Its homologous gene, CHI3L1, has been extensively studied and shown to be related to immune infiltration, indicating a potential role for CHI3L2 in modulating the immune response in tumors .
The preparation of mouse anti-human CHI3L2 involves immunizing mice with human CHI3L2 protein to generate specific antibodies. These antibodies can then be harvested and purified for use in various research applications, including immunohistochemistry (IHC), enzyme-linked immunosorbent assay (ELISA), and western blotting. These techniques are used to study the expression and function of CHI3L2 in different biological contexts, including cancer research and immune response studies.
