CHI3L2 Human, Sf9

CHI3L2 is secreted by various cell types, including synovial fibroblasts, chondrocytes, and TGF-beta/IL-4-stimulated monocyte-derived macrophages . It plays a significant role in cartilage biogenesis and is involved in the regulation of extracellular matrix composition. The protein is also implicated in inflammatory processes and tissue remodeling.

The human recombinant CHI3L2 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 372 amino acids, with a molecular mass of approximately 41.9 kDa . The protein is expressed with an 8 amino acid His tag at the C-terminus and is purified using proprietary chromatographic techniques .

The recombinant CHI3L2 is produced using the Sf9 Baculovirus expression system. This involves infecting Sf9 insect cells with a baculovirus vector containing the CHI3L2 gene. The infected cells then express the CHI3L2 protein, which is subsequently harvested and purified .

CHI3L2 is formulated in a phosphate-buffered saline (PBS) solution with 10% glycerol, maintaining a pH of 7.4 . The protein is stable when stored at 4°C for short-term use (2-4 weeks) and at -20°C for long-term storage. It is recommended to add a carrier protein, such as 0.1% human serum albumin (HSA) or bovine serum albumin (BSA), to prevent degradation during storage .

CHI3L2 is used in various research applications, including studies on cartilage biogenesis, inflammation, and tissue remodeling. Its ability to bind glycans makes it a valuable tool for investigating glycan interactions and their biological implications .