CHI3L1 Human

CHI3L1 was initially identified in the culture supernatant of the osteosarcoma cell line MG63 . It is secreted by a variety of cells, including:

• Articular chondrocytes
• Synovial cells
• Activated monocyte-derived macrophages
• Neutrophils
• Endothelial cells
• Vascular smooth muscle cells
• Some cancer cells

The human CHI3L1 cDNA encodes a protein of 383 amino acids, including a 21 amino acid signal sequence and a 362 amino acid mature region with two intramolecular disulfides . The predicted molecular mass of CHI3L1 is approximately 41.3 kDa, although it typically appears as a 38-41 kDa band under reducing conditions in SDS-PAGE .

CHI3L1 is involved in various biological processes, including:

• Enhancing cell adhesion
• Promoting cell signaling
• Facilitating cell proliferation
• Supporting tumor angiogenesis

It has been found to bind heparins, likely as heparan sulfate, which further contributes to its role in cell adhesion and signaling .

CHI3L1 has been implicated in several diseases and conditions, such as:

• Arthritis
• Asthma
• Inflammatory bowel diseases
• Cancer

Its expression is regulated by various factors, including extracellular matrix changes, microRNAs, growth factors, cytokines, stress, and drugs .

Recombinant human CHI3L1 is typically produced in a mouse myeloma cell line (NS0) and is available in a carrier-free form, which means it does not contain bovine serum albumin (BSA). This is particularly useful for applications where the presence of BSA could interfere with experimental results .

Recombinant CHI3L1 is usually lyophilized from a 0.2 μm filtered solution in PBS and should be reconstituted at 200 μg/mL in PBS. It is stable for 12 months from the date of receipt when stored at -20 to -70 °C as supplied. After reconstitution, it remains stable for 1 month at 2 to 8 °C under sterile conditions and for 3 months at -20 to -70 °C under sterile conditions .