CHI3L1 (22-383) Human
Chitinase 3-Like 1 (22-383 a.a) Human Recombinant
Cat. No.
BT30872
Source
Sf9,
Baculovirus cells.
Synonyms
Chitinase 3 Like 1, Chitinase 3-Like 1 (Cartilage
Glycoprotein-39), Cartilage Glycoprotein 39, 39 KDa Synovial Protein, HCGP-39,
CGP-39, YKL-40, GP-39 , Chitinase-3-Like Protein 1, Cartilage Glycoprotein-39,
HC-Gp39, HCGP-3P, YYL-40, ASRT7, YKL40.
Appearance
Sterile Filtered colorless solution.
Purity
Greater
than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
CHI3L1 produced in Sf9 Baculovirus cells is a single,
glycosylated polypeptide chain containing 370 amino acids (22-383 a.a.) and
having a molecular mass of 41.4kDa (Migrates at 40-57kDa on SDS-PAGE under
reducing conditions).
CHI3L1 is expressed with an 8 amino acid His tag at
C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Introduction
Chitinase 3-Like 1 (CHI3L1) is an enzyme that typically breaks down chitin, a complex sugar found in insect exoskeletons and fungal cell walls. However, human CHI3L1 lacks this chitinase activity. Instead, it plays a role in inflammation and tissue remodeling. Produced by various cells like macrophages, chondrocytes, neutrophils, and synovial cells, CHI3L1 belongs to the glycosyl hydrolase 18 family.
Description
This product consists of the CHI3L1 protein, specifically amino acids 22 to 383 of the human sequence. It's produced using Sf9 insect cells modified with a baculovirus system. The protein is a single chain with glycosylation, weighing approximately 41.4 kDa. When analyzed by SDS-PAGE under reducing conditions, it appears between 40-57 kDa due to the glycosylation. For purification, proprietary chromatographic techniques are used. An 8 amino acid His tag is present at the C-terminus to aid in this process.
Physical Appearance
A clear, colorless liquid without any particles, sterilized by filtration.
Formulation
The CHI3L1 protein is provided at a concentration of 0.25mg per milliliter. It's dissolved in a solution containing Phosphate Buffered Saline at a pH of 7.4, 10% glycerol, and 1mM DTT.
Stability
For optimal short-term storage (2-4 weeks), keep the vial refrigerated at 4°C. For longer periods, store the protein frozen at -20°C. To ensure stability during extended storage, consider adding a carrier protein such as HSA or BSA (0.1% concentration). Repeated freezing and thawing of the product should be avoided.
Purity
Determined by SDS-PAGE analysis, the purity of this CHI3L1 protein is greater than 90%.
Synonyms
Chitinase 3 Like 1, Chitinase 3-Like 1 (Cartilage
Glycoprotein-39), Cartilage Glycoprotein 39, 39 KDa Synovial Protein, HCGP-39,
CGP-39, YKL-40, GP-39 , Chitinase-3-Like Protein 1, Cartilage Glycoprotein-39,
HC-Gp39, HCGP-3P, YYL-40, ASRT7, YKL40.
Source
Sf9,
Baculovirus cells.
Amino Acid Sequence
YKLVCYYTSW SQYREGDGSC FPDALDRFLC THIIYSFANI SNDHIDTWEW
NDVTLYGMLN TLKNRNPNLK TLLSVGGWNF GSQRFSKIAS NTQSRRTFIK SVPPFLRTHG FDGLDLAWLY
PGRGDKQHFT TLIKEMKAEF IKEAQPGKKQ LLLSAALSAG KVTIDSSYDI AKISQHLDFI SIMTYDFHGA
WRGTTGHHSP LFRGQEDASP DRFSNTDYAV GYMLRLGAPA SKLVMGIPTF GRSFTLASSE TGVGAPISGP
GIPGRFTKEA GTLAYYEICD FLRGATVHRI LGQQVPYATK GNQWVGYDDQ ESVKSKVQYL KDRQLAGAMV
WALDLDDFQG SFCGQDLRFP LTNAIKDALA ATLEHHHHHH.
Product Science Overview
Structure and Expression
The recombinant form of Chitinase 3-Like 1 (22-383 a.a) is a fragment of the full-length protein, spanning amino acids 22 to 383 . This fragment is often expressed in E. coli or other expression systems and is tagged with various markers such as His-SUMO or Myc for purification and detection purposes . The recombinant protein is typically purified to a high degree of purity (>95%) and is used in various research applications .
Biological Functions
CHI3L1 is secreted by a variety of cell types, including:
- Articular chondrocytes
- Synovial cells
- Activated monocyte-derived macrophages
- Neutrophils
- Endothelial cells
- Vascular smooth muscle cells
- Certain cancer cells
This protein plays a crucial role in:
- Inflammatory responses: It is involved in the regulation of inflammation and has been implicated in diseases such as asthma, rheumatoid arthritis, and inflammatory bowel disease .
- Tissue remodeling: CHI3L1 is involved in the remodeling of extracellular matrix components, which is essential for wound healing and tissue repair .
- Cancer progression: Elevated levels of CHI3L1 have been associated with various cancers, including breast, lung, and colorectal cancers .
Applications in Research
Recombinant CHI3L1 is widely used in research to study its role in various biological processes and diseases. It is utilized in:
These applications help researchers understand the protein’s function, its interactions with other molecules, and its potential as a therapeutic target.
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