CD86 Antibody, Biotin

CD86, also known as B7-2, B70, and Ly-58, is an 80 kDa glycoprotein that belongs to the immunoglobulin superfamily. It is expressed on the surface of various immune cells, including activated B and T cells, macrophages, dendritic cells, and astrocytes . CD86 plays a crucial role in the immune response by acting as a co-stimulatory molecule that enhances T cell activation and proliferation.

CD86 is a ligand for two important receptors on T cells: CD28 and CTLA-4 (CD152). The interaction between CD86 and CD28 provides a necessary co-stimulatory signal for T cell activation, leading to T cell proliferation and cytokine production . Conversely, the binding of CD86 to CTLA-4 delivers an inhibitory signal that downregulates T cell responses, thus maintaining immune homeostasis .

The biotinylated rat anti-mouse CD86 antibody is a monoclonal antibody that specifically binds to the CD86 molecule on mouse cells. This antibody is conjugated with biotin, a vitamin that can be detected using avidin or streptavidin-based detection systems, making it useful for various immunological assays .

The biotinylated rat anti-mouse CD86 antibody is widely used in research to study the role of CD86 in immune responses. Some common applications include:

• Flow Cytometry: Used to analyze the expression of CD86 on the surface of immune cells .
• Immunohistochemistry: Employed to detect CD86 in tissue sections .
• Blocking Studies: Used to inhibit the interaction between CD86 and its receptors, thereby studying the functional role of CD86 in immune responses .