CD86 Human
Sf9, Baculovirus cells.
B7-2/CD86, CD86, B7-2, B7.2, B70, CD28LG2, LAB72, T-lymphocyte activation antigen CD86, Activation B7-2 antigen, B70, BU63, CTLA-4 counter-receptor B7.2, FUN-1.
Greater than 90% as determined by SDS-PAGE.
Description
CD86 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 223 amino acids (31-247a.a.) and having a molecular mass of 25.6kDa (Molecular size on SDS-PAGE will appear at approximately 40-57kDa). CD86 is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
B7-2/CD86, CD86, B7-2, B7.2, B70, CD28LG2, LAB72, T-lymphocyte activation antigen CD86, Activation B7-2 antigen, B70, BU63, CTLA-4 counter-receptor B7.2, FUN-1.
Sf9, Baculovirus cells.
YFNETADLPC QFANSQNQSL SELVVFWQDQ ENLVLNEVYL GKEKFDSVHS KYMGRTSFDS DSWTLRLHNL QIKDKGLYQC IIHHKKPTGM IRIHQMNSEL SVLANFSQPE IVPISNITEN VYINLTCSSI HGYPEPKKMS VLLRTKNSTI EYDGIMQKSQ DNVTELYDVS ISLSVSFPDV TSNMTIFCIL ETDKTRLLSS PFSIELEDPQ PPPDHIPHHH HHH.
Product Science Overview
CD86 is a type I transmembrane protein consisting of an extracellular domain, a transmembrane domain, and a cytoplasmic tail . The extracellular domain is responsible for binding to its ligands, CD28 and CTLA-4 . The gene encoding CD86 is located on chromosome 3 in humans . The protein is expressed on the surface of APCs and is upregulated upon activation .
The primary function of CD86 is to provide a costimulatory signal necessary for T cell activation and survival . When an APC presents an antigen to a T cell, the interaction between CD86 and CD28 provides the necessary second signal for T cell activation . This interaction leads to the production of cytokines, which are essential for the proliferation and differentiation of T cells . Conversely, the interaction between CD86 and CTLA-4 serves as an inhibitory signal, helping to regulate the immune response and prevent overactivation .
Recombinant CD86 is produced using various expression systems, including E. coli and HEK293 cells . The recombinant protein is often tagged with a polyhistidine tag to facilitate purification . It is used in various research applications, including studying T cell activation, immune regulation, and developing immunotherapies .
Recombinant CD86 is widely used in immunological research to study the mechanisms of T cell activation and regulation . It is also used in the development of immunotherapies for diseases such as cancer and autoimmune disorders . By understanding the role of CD86 in the immune response, researchers can develop targeted therapies to modulate the immune system effectively .
